Mouse CD152/CTLA-4 Antibody : Biotin
Référence OASB01723
Conditionnement : 0.5mg
Marque : Aviva Systems Biology
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Mouse CD152/CTLA-4 Antibody : Biotin (OASB01723)
| Datasheets/Manuals | Printable datasheet for Mouse CD152/CTLA-4 Antibody : Biotin (OASB01723) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | 1B8 |
| Isotype | IgG |
| Host | Hamster |
| Conjugation | Biotin |
| Application | FC |
| Additional Information | Description: The lymphocyte surface antigen CD152, also known as CTLA-4, is related to the costimulatory molecule CD28 and both molecules share common B7 family counter-receptors. However CD152 is thought to be a negative regulator of T cell activation and may play a role in apoptotic control of T cells. CD152 is relatively conserved among humans, mice, and chickens. |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Extracellular portion of murine CTLA-4 fused to a murine IgG2a |
| Concentration | 0.5 mg/mL |
| Specificity | CD152 |
| Characterization | To insure acceptable performance, each batch of product is tested to conform to the characteristics of astandard reference reagent by flow cytometry. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate <= 3 ug/106 cells <= 3 ug/106 cells <= 0.3 ug/106 cells |
| Application Info | Flow cytometry 5, In vitro functional studies 5 |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Other Applications Image 1 Data | Amount Used: ≤ 3 Ug/106 cells CHO cells transfected with CD152/CTLA-4 were stained with either hamster IgG-FITC or hamster anti-mouse CD152-FITC, following which large cells were gated and analyzed by flow cytometry. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Walunas, T. L., et al. 1994. Immunity 1:405 2. Perkins, D., et al. 1996. J. Immunol. 156:4154 3. Leach, D. R., M.K. Krummel and J.P. Allison. 1996. Science 271:1734 4. Freeman, G. J., et al. 1992. J. Immunol. 149:3795 5. Ben- David, H., M. Sela, and E. Mozes. 2005. Proc. Natl. Sci. USA 102(6):2028. |
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| Gene Symbol | CTLA4 |
| Gene Full Name | Cytotoxic T-lymphocyte protein 4 |
| Alias Symbols | Ctla, Ly-5, Cd152, Ly-56, Ctla-4 |
| NCBI Gene Id | 12477 |
| Protein Name | cytotoxic T-lymphocyte protein 4 |
| Description of Target | This gene is a member of the immunoglobulin superfamily, and encodes a protein that functions as a negative regulator of T-cell responses. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. |
| Uniprot ID | P09793 |
| Protein Accession # | NP_033973.2 |
| Nucleotide Accession # | NM_009843.4 |
