Mouse CD3e Antibody : CY5
Référence OASB00255
Conditionnement : 0.1mg
Marque : Aviva Systems Biology
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Mouse CD3e Antibody : CY5 (OASB00255)
| Datasheets/Manuals | Printable datasheet for Mouse CD3e Antibody : CY5 (OASB00255) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | C363.29B |
| Isotype | IgG2b |
| Host | Rat |
| Conjugation | Cyanine 5 |
| Application | FC, IHC-F, IHC-P |
| Additional Information | Description: CD3 epsilon, a member of the immunoglobulin superfamily of cell surface receptors, is comprised of five invariable chain ranging in size from 16-28 kDa and is closely associated with the T cell antigen receptor (TCR). It is expressed on all T cells of all mouse strains. CD3 plays a major role in signaling during antigen recognition, leading to T-cell activation. The monoclonal antibody C363.29B recognizes an epitope on the 25 kDa epsilon chain of the CD3/TCR complex. In the presence of Fc receptor-bearing accessory cells, soluble C363.29B can activate primed and naive T cell in vitro. Immobilized C363.2B9 monoclonal antibody can also activate both normal T lymphocytes and cloned T cell lines provided the appropriate accessory signals are present. The monoclonal antibody is cytolytic, easily used for cell surface staining, and a good immunoprecipitating antibody. |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | IL-4 producing Th2 cell lines including D10 |
| Concentration | 0.1 mg/mL |
| Specificity | CD3e |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested to conform to characteristicsof a standard reference reagent by flow cytometry. Representative data are included in this data sheet. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Fluorescein conjugate <= 3 ug/106 cells Biotin conjugate <= 3 ug/106 cells R-phycoerythrin conjugate <= 1 ug/106 cells Allophycocyanin conjugate <= 1 ug/106 cells Spectral Red conjugate <= 1 ug/106 cells Cyanine 5 conjugate <= 1 ug/106 cells |
| Application Info | Identification and enumeration of CD3+ cells by flow cytometry 1, 2, Immunoprecipitation 1, In vitro depletion of CD3+ cells 1, In vitro activation of T cells (accessory cells must be provided) 1, 3, 4, Immunohistochemistry (PS)5 |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution for the product that is appropriate for your specific need. |
| Other Applications Image 1 Data | Amount Used: ≤ 3 Ug/106 Cells from BALB/c mesenteric lymph nodes were double-stained with anti-mouse CD3epsilon-FITC and anti-mouse CD45R/B220-R-PE , gated on small lymphocytes and analyzed using a FACScan flow cytometer (BDIS, San Jose, CA). |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Portoles, P., J. Rojo, A. Golby, M. Bonneville, S. Gromkowski, L. Greenbaum, C. A. Janeway, Jr., D. B. Murphy, and K. Bottomly. 1989. J Immunol. 142:4169 2. Dianzani, U., M. Luqman, J. Rojo, J. Yagi, J. L. Baron, A. Woods, C. A. Janeway, Jr., and K. Bottomly. 1990. Eur. J. Immunol. 20:2249 3. Luqman, M., L. Greenbaum, D. Lu, and K. Bottomly. 1992. Eur. J. Immunol. 22:95 4. Ernst, D. N., M. V. Hobbs, B. E. Torbett, A. L. Glasebrook, M. A. Rehse, K. Bottomly, K. Hayakawa, R. R. Hardy, and W. O. Weigle. 1990 J. Immunol. 145:1295 5. Whiteland, J.L., S. M. Nicholls, C. Shimeld, D.L. Easty, N. A. Williams, and T. J. Hilli. 1995. J. Histochem Cytochem 43(3):313- 320. |
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| Gene Symbol | CD3E |
| Gene Full Name | T-cell surface glycoprotein CD3 epsilon chain |
| Alias Symbols | CD3, T3e, AI504783, CD3epsilon |
| NCBI Gene Id | 12501 |
| Protein Name | T-cell surface glycoprotein CD3 epsilon chain |
| Uniprot ID | P22646 |
| Protein Accession # | NP_031674.1 |
| Nucleotide Accession # | NM_007648.4 |
