Mouse CD45R/B220 Antibody : APC/CY7
Référence OASB00471
Conditionnement : 0.1mg
Marque : Aviva Systems Biology
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Mouse CD45R/B220 Antibody : APC/CY7 (OASB00471)
| Datasheets/Manuals | Printable datasheet for Mouse CD45R/B220 Antibody : APC/CY7 (OASB00471) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | RA3-6B2 |
| Isotype | IgG2a |
| Host | Rat |
| Conjugation | APC-Cyanine 7 |
| Application | FC |
| Additional Information | Description: CD45R, also known as B220, a member of the protein tyrosine phosphate family and a major cell surface glycoprotein, represents a restricted form of the CD45 family which primarily recognizes only cells of B-lineage from pro-B cell through mature B lymphocytes and prior to the availability of anti-CD19 monoclonal antibodies was commonly used as a pan B-cell marker. It also reacts with certain activated T cells as well as non-MHC-restricted lytically active lymphokine-activated killer (LAK) cells. In vivo administration of RA3-6B2 has been shown to affect differentiation of both T and B cells in normal mice and reduce the level of anti-DNA antibodies and lymphadenopathies in MRL/lpr mice. |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Abelson murine leukemia virus-induced pre-B cell lymphoma |
| Concentration | 0.1 mg/mL |
| Specificity | CD45R |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. Representative data are included in this product insert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin, eyes, or clothing.Wear eye or face protection when handling. If skin or eye contact occurs, wash with copious amounts of water. If ingestedor inhaled, contact a physician immediately. Sodium azide yields toxic hydrazoic acid under acidic conditions. Dilute azidecontainingcompounds in running water before discarding to avoid accumulation of potentially explosive deposits in lead orcopper plumbing. |
| Dilution | Flow Cytometry: Fluorescein conjugate <= 1 ug/106 cells Biotin conjugate <= 1 ug/106 cells R-phycoerythrin conjugate <= 0.2 ug/106 cells R-phycoerythrin conjugate-Texas Red® <= 0.2 ug/106 cells Allophycocyanin conjugate <= 0.2 ug/106 cells Spectral Red conjugate <= 0.2 ug/106 cells Cyanine 5 conjugate <= 0.2 ug/106 cells R-phycoerythrin-Cyanine 5.5 conjugate <= 0.2 ug/106 cells R-phycoerythrin-Cyanine 7 conjugate <= 0.2 ug/106 cells Allophycocyanin-Cyanine 5.5 conjugate Allophycocyanin-Cyanine 7 conjugate Alexa Fluor 488 <= 0.2 ug/106 cells <= 0.2 ug/106 cells <= 0.2 ug/106 cells |
| Application Info | Flow cytometry 9,10, Immunohistochemistry (frozen sections) 10 |
| Other Applications Data | Since applications vary, you should determine the optimum working dilution for theproduct that is appropriate for your specific need. |
| Other Applications Image 1 Data | Amount Used: 0.3 Ug/106 cells Cells from BALB/c mesenteric lymph nodes were double-stained with rat anti-mouse CD45/B220-PE and rat anti-mouse CD3epsilon-FITC. Small lymphocytes were then gated and analyzed on a FACScan flow cytometer (BDIS, San J |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purifiedimmunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. Nopreservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg or 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3 and astabilizing agent. Store at 2- 8 C. Do not freeze! - The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at2- 8 C. Do not freeze! - The Spectral Red (SPRD), R- phycoerythrin - Texas Red (R- PE- TXRD), R- phycoerythrin- Cyanine 7 (R- PE- CY 7),R- phycoerythrin- Cyanine 5.5 (R- PE- CY 5.5), Allophycocyanin- Cyanine 5.5 (APC- CY 5.5) and Allophycocyanin- Cyanine 7 (APC- CY 7) conjugates are supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquotand store at or below - 20 C - The Cyanine 5 (CY 5) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The Alexa Fluor 488 (AF488) conjugate is supplied as 0.1mg in 0.2mL of PBS/NaN3. Store at 2- 8 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 C immediately uponreceipt. Each reagent is stable for the period shown on the bottle label if stored as directed. |
| Reference | 1. Coffman, R. L. 1982. Immunol. Rev. 69:5 2. Ballas, Z. K. and W. Rasmussen. 1990. J. Immunol. 144:386 3. Morse, H. C. III, W. F. Davidson, R. A. Yetter, and R. L. Coffman. 1982. Cell Immunol. 70:311 4. Johnson, P., L. Greenbaum, K. Bottomly, and I. S. Trowbridge. 1989. J. Exp. Med. 169:1179 5. Kincade, P. W., L. Grace, W. Takeshi, S. Leslie, and M. P. Scheid. 1981. J. Immunol. 127:2262 6. Watanabe, Y. and T. Akaike. 1994. Scand. J. Immunol. 39:419 7. Asensi, V., K. Himeno, I. Kawamura, M. Sakumoto, and K. Nomoto. 1990. Clin. Exp. Immunol. 80:268 8. Asensi, V., K. Kimeno, I. Kawamura, M. Sakumoto, and K. Nomoto. 1989. Immunology 68:204 9. Hardy, R. R., C. E. Carmack, S. A. Shinton, J. D. Kemp, and K. Hayakawa. 1991. J. Exp. Med 173:1213 10. Ermak, T.H., P.J. Giannasca, R. Nichols, G.A. Myers, J. Nedrud, R. Weltzin, C.K. Lee, H. Kleanthous, and T.P. Monath. 1998. J. Exp. Med 188(12):2277. |
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| Gene Symbol | PTPRC |
| Gene Full Name | Receptor-type tyrosine-protein phosphatase C |
| Alias Symbols | Ly-, loc, B220, Cd45, L-CA, Ly-5, Lyt-, T200, CD45R, Lyt-4 |
| NCBI Gene Id | 19264; 5788 |
| Protein Name | receptor-type tyrosine-protein phosphatase C |
| Uniprot ID | P06800, P08575 |
| Protein Accession # | NP_001104786.2 |
| Nucleotide Accession # | NM_001111316.2 |
