Mouse CD49b/VLA-2a Antibody : LE/AF
Référence OASB00619
Conditionnement : 0.5mg
Marque : Aviva Systems Biology
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Mouse CD49b/VLA-2a Antibody : LE/AF (OASB00619)
| Datasheets/Manuals | Printable datasheet for Mouse CD49b/VLA-2a Antibody : LE/AF (OASB00619) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | DX5 |
| Isotype | IgM |
| Host | Rat |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | FC, IHC-P, IHC-F |
| Additional Information | Description: The monoclonal antibody DX5 reacts with CD49b, also known as very late antigen-2 (VLA-2) and alpha2 integrin. The antibody stains the majority of NK cells and a small subpopulation of T cells in all mouse strains tested (e.g., A/J, AKR, BALB/c, C3H/HeJ, C57BL/6, C57BL/10, C57BR, C58, CBA/Ca, CBA/J, DBA/1, DBA/2, SJL, SWR). Three-color flow cytometry analysis has revealed that most cells that express NK-1.1 (NKR-P1C) also express CD49b. However, small subsets of DX5+NK-1.1- and DX5-NK-1.1+ cells can be found, especially among the CD3+ population of T lymphocytes. NK cells cultured in the presence of IL-2 progressively lose reactivity with DX5 as a consequence of cellular proliferation. Therefore, DX5 can be used to define functionally distinct subsets of murine NK cells. The monoclonal antibody DX5 has not been demonstrated to have activating or blocking activity. |
| Reconstitution and Storage | Store at -20C |
| Immunogen | NK cells isolated from C57BL/6 mice |
| Concentration | 0.5 mg/mL |
| Specificity | CD49b |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conformto characteristics of a standard reference reagent. Representative data are included in this product insert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate Allophycocyanin conjugate Allophycocyanin-Cyanine 7 (APC-CY7) conjugate <= 1 ug/106 cells <= 1 ug/106 cells <= 0.2 ug/106 cells <= 0.2 ug/106 cells <= 0.2 ug/106 cells |
| Application Info | Flow cytometry 1-4 |
| Other Applications Data | Since applications vary, investigators are advised to determine the optimalworking dilution of the product that is appropriate for a specific need. |
| Other Applications Image 1 Data | Amount Used: 1 Ug/106 cells Splenocytes from a C57BL/6 SCID mouse were doublestained with rat anti-mouse CD49b-FITC and mouse antimouse NK1.1-R-PE. Lymphocytes were gated using light scatter criteria and analyzed on a FACScan flow cytometer (BDIS |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The Allophycocyanin- Cyanine 7 (APC- CY 7) conjugates are supplied as 0.1 mg in 1.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Arase, H., T. Saito, J.H. Phillips, and L.L. Lanier. 2001. J. Immunol. 167:1141- 1144 2. Abrams, J. Personal communication3. Moore, T.A., U. von Freeden- Jeffry, R. Muray, and A. Zlotnik. 1996. J. Immunol. 157:2366- 2373 4. Pellicci, D.G., K.L. Hammond, J. Coquet, K. Kyparissoudis, A.G. Brooks, K. Kedzierska, R. Keating, S. Turner, S. Berzins, M.J. Smith, and. D. I. Godfrey. 2005. J. Immunol. 175:4416. |
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| Gene Symbol | ITGA2 |
| Gene Full Name | Integrin alpha-2 |
| Alias Symbols | DX5, GPIa, CD49B |
| NCBI Gene Id | 16398 |
| Protein Name | integrin alpha-2 |
| Uniprot ID | Q62469 |
| Protein Accession # | NP_032422.2 |
| Nucleotide Accession # | NM_008396.2 |
