Mouse CD69/VEA Antibody : LE/AF
Référence OASB01700
Conditionnement : 0.5mg
Marque : Aviva Systems Biology
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Mouse CD69/VEA Antibody : LE/AF (OASB01700)
| Datasheets/Manuals | Printable datasheet for Mouse CD69/VEA Antibody : LE/AF (OASB01700) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | H1.2F3 |
| Isotype | IgG |
| Host | Hamster |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | FC, IHC-F, IP |
| Additional Information | Description: CD69, also known as very early activation (VEA) antigen, is a disulfide-linked transmembrane homodimer whose differentially glycosylated subunits range from 35-39 kDa. It is a C-type lectin most closely related to the NKR-P1 and Ly-49 NK cell-activation molecules. CD69 is widely expressed on hematopoietic cells, including lymphocytes, neutrophils and eosinophils. Although not detectable on resting lymphocytes, its expression is rapidly upregulated upon activation of T, B and NK cells, and neutrophils. Constitutive expression of CD69 on subsets of thymocytes suggests that it may be involved in regulation of developmental events in addition to its role in activation of a variety of hematopoietic cells. The monoclonal antibody H1.2F3 augments PMA-induced T-cell proliferation and induces redirected lysis of Fc receptor-bearing target cells by NK cells. |
| Reconstitution and Storage | Store at -20C |
| Immunogen | Mouse dendritic epidermal cell line Y245 |
| Concentration | 0.5 mg/mL |
| Specificity | CD69 |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. Representative data are included in this product insert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yields toxichydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water before discardingto avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Fluorescein conjugate <= 1 ug/106 cells Biotin conjugate <= 1 ug/106 cells R-phycoerythrin conjugate <= 0.2 ug/106 cells Allophycocyanin conjugate <= 0.2 ug/106 cells Spectral Red conjugate <= 0.2 ug/106 cells R-phycoerythrin-Cyanine 7 conjugate <= 0.2 ug/106 cells |
| Application Info | Flow cytometry 1,2,4-7, Immunoprecipitation 1, In vitro T cell and NK-cell activation 1,7 |
| Other Applications Data | Since applications vary, you should determine the optimum working dilutionfor the product that is appropriate for your specific need. |
| Other Applications Image 1 Data | Amount Used: ≤1 Ug/106 cells Fresh BALB/c spleen cells were activated by incubation for 20 hours with PMA + ionomycin, then stained with either hamster IgG-FITC (as an isotype control) or hamster anti-mouse CD69-FITC. Large, activated cells were |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL of PBS/NaN3and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The allophycocyanin (APC) conjugate is supplied as 100 tests in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD) and R- phycoerythrin- Cyanine 7 (R- PE- CY 7) conjugates are supplied as0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL ofPBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Yokoyama, W. M., F. Koning, P. J. Kehn, G. M. Pereira, G. Stingl, J. E. Coligan, and E. M. Shevach. 1988. J. Immunol. 141:369 2. Yokoyama, W.M., S.R. Maxfield, and E.M. Shevach. 1989. Immunol. Rev. 109:153 3. Zeigler, S.F., S.D. Levin, L. Johnson, N.G. Copeland, et al. 1994. J. Immunol. 152:1228 4. Ziegler, S.F., F. Ramsdell, and M.R. Alderson. 1994. Stem Cells 12:456 5. Brandle, D., S. Muller, C. Muller, H. Hengartner, and H. Pircher. 1994. Eur. J. Immunol. 24:145 6. Sobel, E. S., W. M. Yokoyama, E. M. Shevach, R. A. Eisenberg, and P. L. Cohen. 1993. J. Immunol. 150:673 7. Karlhofer, F. M. and W. M. Yokoyama. 1991. J. Immunol. 146:3662. |
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| Gene Symbol | CD69 |
| Gene Full Name | Early activation antigen CD69 |
| Alias Symbols | A, V, AIM, VEA, AI452015, 5830438K24Rik |
| NCBI Gene Id | 12515 |
| Protein Name | early activation antigen CD69 |
| Uniprot ID | P37217 |
| Protein Accession # | NP_001028294.1 |
| Nucleotide Accession # | NM_001033122.3 |
