Mouse CD86/B7-2 Antibody : APC
Référence OASB00732
Conditionnement : 0.1mg
Marque : Aviva Systems Biology
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Mouse CD86/B7-2 Antibody : APC (OASB00732)
| Datasheets/Manuals | Printable datasheet for Mouse CD86/B7-2 Antibody : APC (OASB00732) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | 2D10 |
| Isotype | IgG2b |
| Host | Rat |
| Conjugation | APC |
| Application | FC |
| Additional Information | Description: CD86 (B7-2) is an 80 kDa type I integral membrane protein and a member of the immunoglobulin superfamily of cell surface receptors. Along with CD80 (B7-1), CD86 participates in T cell costimulation via interaction with CD28 and CD152 (CTLA-4). It is expressed at very low density in normal tissues but is rapidly upregulated on stimulated B cells, monocytes, and dendritic cells. Since CD86 has rapid kinetics of induction, it is believed to be the major CD28 ligand expressed early in the immune response. |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | Mouse B cell lymphoma cell line 5C2 |
| Concentration | 0.1 mg/mL |
| Specificity | CD86 |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conformto characteristics of a standard reference reagent. Representative data are included in this product insert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate Allophycocyanin conjugate Spectral Red conjugate R-phycoerythrin-Cyanine 7 conjugate < 1 ug/106 cells < 1 ug/106 cells < 0.2 ug/106 cells < 0.2 ug/106 cells < 0.2 ug/106 cells < 0.2 ug/106 cells |
| Application Info | Identification and enumeration of CD86+ cells by flow cytometry 9, Immunohistochemistry (Frozen sections) 9, In vivo and in vitro blocking assays 10,11 |
| Other Applications Data | Since applications vary, each investigator must determine thedilutions appropriate for their individual needs. |
| Other Applications Image 1 Data | Amount Used: u1 ug/106 cells |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg ofpurified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives oramine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The allophycocyanin (APC) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD) and R- phycoerythrin- Cyanine 7 (R- PE- CY 7) conjugates are supplied as0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. Hathcock, K., G. Laszlo, C. Pucillo, P. Linsley, and R.J. Hodes. 1994. J. Exp. Med. 180:631 2. Freeman, G.J., F. Borriello, R.J. Hodes, H. Reiser, K.S. Hathcock, G. Laszlo, A.J. McKnight, J. Kim, L. Du, D.B. Lombard, G.S. Gray, L.M. Nadler, and A.H. Sharpe. 1993. Science 262:907 3. Hathcock, K., G. Laszlo, H. Dickler, J. Bradshaw, P. Linsley, and R Hodes. 1993. Science 262:905 4. Thompson, C.B. 1995. Cell Jun 30; 81(7) 979- 82 5. June, C.H., J.A. Bluestone, L.M. Nadler, and C.B. Thompson. 1994. Immuol. Today 15:321 6. Han, S. et al. 1995. J. Immunol. 155:556 7. Inaba, K. et al. 1994. J. Exp. Med. 180:1849 8. Larsen, C.P., et al. 1994. J. Immunol. 152:5208 9. Issazadeh, S., V. Navikas, M. Schaub, M. Sayegh, and S. Khoury. 1998. J. Immunol. 161:1104 10. Shanafelt, M., I. Kang, S.W. Barthold, and L.K. Bockenstedt. 1998. Inf. and Immun. 66(1):266 11. Chitnis, T., N. Najafian, K.A. Abdallah, V. Dong, H. Yagita, M.H. Sayegh, and S.J. Khoury. 2001. J. Clin. Invest. 107:575. |
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| Gene Symbol | CD86 |
| Gene Full Name | T-lymphocyte activation antigen CD86 |
| Alias Symbols | B7, B70, Ly5, MB7, B7-2, B7.2, CLS1, Ly-5, Ly58, Cd28l, ETC-1, Ly-58, MB7-2, Cd28l2, TS/A-2 |
| NCBI Gene Id | 12524 |
| Protein Name | T-lymphocyte activation antigen CD86 |
| Uniprot ID | P42082 |
| Protein Accession # | NP_062261.3 |
| Nucleotide Accession # | NM_019388.3 |
