RayBio® G-Series Mouse Cytokine Antibody Array 2000. A combination of Mouse Cytokine Antibody Array G3, G4, & G5 Kit. Detects 144 Mouse Cytokines. Suitable for all liquid sample types.
Product Description
Specifications
| Size | 4 Sample Kit, 8 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Semi-Quantitative |
| Number of Targets Detected | 144 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- High sensitivity and specificity
- Low sample volume (10-100 µl per array)
- Large dynamic range of detection
- Compatible with most sample types
- Test 4 or 8 samples on each slide
- Suitable for high-throughput assays
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| 4-1BB (TNFRSF9/CD137)
| 6Ckine (CCL21)
| ACE
| ALK-1
| Amphiregulin
|
| Axl
| bFGF
| BLC (CXCL13)
| Cardiotrophin-1 (CT-1)
| CD27 (TNFRSF7)
|
| CD27 Ligand (TNFSF7)
| CD30 Ligand (TNFSF8)
| CD30 (TNFRSF8)
| CD36 (SR-B3)
| CD40 (TNFRSF5)
|
| CD40 Ligand (TNFSF5)
| Chordin
| CRG-2
| CTACK (CCL27)
| CTLA-4 (CD152)
|
| CXCL16
| Decorin
| DKK-1
| CD26 (DPPIV)
| Dtk
|
| E-Cadherin
| EGF
| Endoglin (CD105)
| Eotaxin-1 (CCL11)
| Eotaxin-2 (MPIF-2/CCL24)
|
| Epigen
| Epiregulin
| E-Selectin
| Fas Ligand (TNFSF6)
| Fc gamma RIIB (CD32b)
|
| Flt-3 Ligand
| Fractalkine (CX3CL1)
| Galectin-1
| GCSF
| GITR (TNFRSF18)
|
| GITR Ligand (TNFSF18)
| GM-CSF
| Granzyme B
| Gas 1
| Gas 6
|
| HAI-1
| HGF
| HGFR
| ICAM-1 (CD54)
| IFN-gamma
|
| IGFBP-2
| IGFBP-3
| IGFBP-5
| IGFBP-6
| IGF-1
|
| IGF-2
| IL-1 beta (IL-1 F2)
| IL-1 R4 (ST2)
| IL-10
| IL-11
|
| IL-12 p40/p70
| IL-12 p70
| IL-13
| IL-15
| IL-17A
|
| IL-17B
| IL-17 RB
| IL-17E (IL-25)
| IL-17F
| IL-1 alpha (IL-1 F1)
|
| IL-1 RA (IL-1 F3)
| IL-2
| IL-2 R alpha
| IL-20
| IL-21
|
| IL-28A (IFN-lambda 2)
| IL-3
| IL-3 R beta
| IL-4
| IL-5
|
| IL-6
| IL-6 R
| IL-7
| IL-9
| I-TAC (CXCL11)
|
| JAM-A (CD321/F11R)
| KC (CXCL1)
| Leptin
| Leptin R
| LIX
|
| L-Selectin (CD62L)
| Lungkine (CXCL15)
| Lymphotactin (XCL1)
| MAdCAM-1
| MCP-1 (CCL2)
|
| MCP-5
| M-CSF
| MDC (CCL22)
| MFG-E8
| MIG (CXCL9)
|
| MIP-1 alpha (CCL3)
| MIP-1 gamma
| MIP-2
| MIP-3 beta (CCL19)
| MIP-3 alpha (CCL20)
|
| MMP-2
| MMP-3
| Neprilysin
| Osteopontin (SPP1)
| Osteoprotegerin (TNFRSF11B)
|
| Pentraxin-3 (TSG-14)
| Platelet Factor 4 (CXCL4)
| Prolactin
| Pro-MMP-9
| P-Selectin
|
| RAGE
| RANTES (CCL5)
| Resistin
| SCF
| SDF-1 alpha (CXCL12 alpha)
|
| Sonic Hedgehog N-Terminal (Shh-N)
| TNF RI (TNFRSF1A)
| TNF RII (TNFRSF1B)
| TACI (TNFRSF13B)
| TARC (CCL17)
|
| I-309 (TCA-3/CCL1)
| TECK (CCL25)
| TCK-1 (CXCL7)
| TIMP-1
| TIMP-2
|
| TNF alpha
| Thrombopoietin (TPO)
| TRANCE (TNFSF11)
| TREM-1
| TROY (TNFRSF19)
|
| TSLP
| TWEAK (TNFSF12)
| TWEAK R (TNFRSF12)
| VCAM-1 (CD106)
| VEGF-A
|
| VEGFR1
| VEGFR2
| VEGFR3
| VEGF-D
| |
Species Detected
Mouse
Application Notes
Suggested Application
Multiplexed Protein Detection; Detection of Relative Protein Expression; Detecting Patterns of Cytokine Expression; Biomarker Screening; Identifying Key Factors; Confirming a Biological Process
Kit Components
- Mouse Cytokine Antibody Array G2000 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Lysis Buffer
- Adhesive Plastic Strips
- 30 ml Centrifuge Tube
- Manual
Other Materials Required
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Block array surface
- Incubate with Sample
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s) and 1X Blocking Buffer at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-AAM-CYT-G3-4.gal)
GAL (GAL-AAM-CYT-G3-8.gal)
GAL (GAL-AAM-CYT-G4-4.gal)
GAL (GAL-AAM-CYT-G4-8.gal)
GAL (GAL-AAM-CYT-G5-4.gal)
GAL (GAL-AAM-CYT-G5-8.gal)
Excel (AAM-CYT-G3_Analysis_Tool.xls)
Excel (AAM-CYT-G4_Analysis_Tool.xls)
Excel (AAM-CYT-G5_Analysis_Tool.xls)
citations
Extracellular vesicles play a central role in linking podocyte injury to mesangial activation in glomerular disease
- Species:
- Mouse
- Sample Type:
- Extracellular Vesicles (EVs from MPC5 cells)
- Year:
- 2025
- Journal Name:
- Theranostics
- PubMed ID:
- 40303345
ID4-dependent secretion of VEGFA enhances the invasion capability of breast cancer cells and activates YAP/TAZ via integrin β3-VEGFR2 interaction
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (conditioned medium from confluent cells)
- Year:
- 2024
- Journal Name:
- Cell Death & Disease
- PubMed ID:
- 38321003
Obesity alters the lung myeloid cell landscape to enhance breast cancer metastasis through IL5 and GM-CSF
- Species:
- Mouse
- Sample Type:
- Serum (Obese and normal mouse models)
- Year:
- 2017
- Journal Name:
- Nature Cell Biology
- PubMed ID:
- 28737771
Increased MFG-E8 at neuromuscular junctions is an exacerbating factor for sarcopenia-associated denervation
- Species:
- Mouse
- Year:
- 2021
CXCR2 Inhibition Profoundly Suppresses Metastases and Augments Immunotherapy in Pancreatic Ductal Adenocarcinoma
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Pancreatic Cancer cells)
- Year:
- 2016
- Journal Name:
- Cancer Cell
- PubMed ID:
- 27265504
PTH Signaling in Osteoprogenitors Is Essential for B-Lymphocyte Differentiation and Mobilization
- Species:
- Mouse
- Sample Type:
- Bone Marrow Aspirate (Mone marrow supernates from control and Osx-PPR KO mice)
- Year:
- 2015
- Journal Name:
- Journal of Bone and Mineral Research
- PubMed ID:
- 26191777
Anti-inflammatory peptides from cardiac progenitors ameliorate dysfunction after myocardial infarction
- Species:
- Mouse
- Sample Type:
- Conditioned Media (Cardiac progenitor cells, Skeletal myobalstas, and adipose-derived mesenchymal cells)
- Year:
- 2014
- Journal Name:
- Journal of the American Heart Association
- PubMed ID:
- 25468657
Pro-Insulin-Like Growth Factor-II Ameliorates Age-Related Inefficient Regenerative Response by Orchestrating Self-Reinforcement Mechanism of Muscle Regeneration
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (tibialis anterior tissue lysate)
- Year:
- 2015
- Journal Name:
- Stem Cells
- PubMed ID:
- 25917344


