Mouse Human IgG1 (Fc subclass specific), conjugated with Horseradish peroxidase Antibody

Référence orb22255-0.2mg

Conditionnement : 0.2mg

Marque : Biorbyt

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Mouse Human IgG1 (Fc subclass specific), conjugated with Horseradish peroxidase Antibody

SKU: orb22255

Description

Peroxidase conjugated purified monoclonal mouse antibody to human IgG1, subclass specific. To identify the presence of IgG1 in human serum, other body fluids, cell and tissue substrates and to determine its concentration in techniques as ELISA, direct immunoperoxidase staining of cytoplasmic IgG1, and immunoblotting. The optimum working dilution is an assay-related characteristic. It may vary widely and should always be determined by titration. For histochemical use optimum dilutions are mostly from 1:10 to 1:40; in ELISA from 1:125 to 1:8,000; in Western blotting from 1:250 to 1:10.000. These data should be interpreted as general recommendations only.

Images & Validation

ReactivityHuman

Key Properties

Antibody TypeSecondary Antibody
HostMouse
ClonalityMonoclonal
IsotypeIgG1
Clone No.NI 132 (HP 6186)
ConjugationHRP

Storage & Handling

StorageThe lyophilized product is shipped at ambient temperature and may be stored at +4°C; prolonged storage at or below -20°C. Dilutions may be prepared by adding phosphate buffered saline (PBS, pH 7.2). Avoid repeated thawing and freezing. If a slight precipitation occurs upon storage, this should be removed by centrifugation and will not affect the performance of the product. Diluted solutions should be stored at +4°C, not refrozen, and preferably used the same day.
Form/AppearancePurified monoclonal mouse IgG1 kappa conjugated with horseradish peroxidase, lyophilized from a solution in phosphate buffered saline (pH7.2). No preservative added, as it may interfere with the antibody activity. No foreign protein added.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only