Mouse I-Ab Antibody : Biotin
Référence OASB00672
Conditionnement : 0.5mg
Marque : Aviva Systems Biology
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Mouse I-Ab Antibody : Biotin (OASB00672)
| Datasheets/Manuals | Printable datasheet for Mouse I-Ab Antibody : Biotin (OASB00672) |
|---|
| Tested Species Reactivity | Mouse |
|---|---|
| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | 25-9-3 |
| Isotype | IgM |
| Host | Mouse |
| Conjugation | Biotin |
| Application | FC, IHC-F |
| Additional Information | Description: The monoclonal antibody 25-9-3 reacts specifically with the I-Ab haplotype of MHC Class II molecules. Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells, and certain epithelial cells. |
| Reconstitution and Storage | Store at 2-8C |
| Immunogen | C3H.SW mouse splenocytes |
| Concentration | 0.5 mg/mL |
| Specificity | I-Ab |
| Characterization | To ensure lot- to- lot consistency, each batch of monoclonal antibody is tested by flow cytometry to conformto characteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate <=1 ug/106 cells <=1 ug/106 cells <=0.2 ug/106 cells |
| Application Info | Flow cytometry3, Immunohistochemistry2 |
| Other Applications Data | Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specificneeds. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label ifstored as directed. |
| Reference | 1. Ozato, K., and D.H. Sachs. 1981. J. Immunol. 126:317- 321 2. Komano,H., Y. Fujiura, M. Kawaguchi, S. Matsumoto, Y. Hashimoto, S. Obana, P. Mombaerts, S. Tonegawa, H. Yamamoto, S. Itohara, M .. Nanno, and H. Ishikawa. 1995. Proc. Natl. Acad. Sci. USA. 92:6147 3. Aviva Systems Biology, Inc. Unpublished observations. |
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| Gene Symbol | H2-AB1 |
| Gene Full Name | H-2 class II histocompatibility antigen, A-Q beta chain |
| Alias Symbols | I, Ia, Abe, IAb, Ia2, Rmc, H-2A, H2-A, I-A<, I-Ab, Ia-2, Abeta, H-2Ab, H2-Ab, Rmcs1, H2-Ab_, I-Abeta, AI845868 |
| NCBI Gene Id | 14961 |
| Protein Name | histocompatibility 2, class II antigen A, beta 1 |
| Uniprot ID | P14483 |
| Protein Accession # | NP_996988.2 |
| Nucleotide Accession # | NM_207105.3 |
