Mouse I-Ek Antibody : LE/AF
Référence OASB00678
Conditionnement : 0.5mg
Marque : Aviva Systems Biology
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Mouse I-Ek Antibody : LE/AF (OASB00678)
| Datasheets/Manuals | Printable datasheet for Mouse I-Ek Antibody : LE/AF (OASB00678) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | 14-4-4S |
| Isotype | IgG2a |
| Host | Mouse |
| Conjugation | LE: Low Endotoxin, AF: Azide Free |
| Application | FC, IHC-F, ICC, IP, CMCD, BL |
| Additional Information | Description: The monoclonal antibody 14-4-4S reacts with the I-Ek class II alloantigen on cells from mice of the H-2d, H-2p, and H-2r haplotypes. Cells from mice of the H-2b, H-2f, H-2q, and H-2s haplotypes do not express I-E antigen. The antibody has been reported to cross-react with the rat MHC class II alloantigen RT1D. |
| Reconstitution and Storage | Store at -20C |
| Immunogen | C3H mouse skin graft and splenocytes |
| Concentration | 0.5 mg/mL |
| Specificity | I-Ek|RT1D |
| Characterization | To ensure acceptable performance, each batch of product is tested by flow cytometry to conform to thecharacteristics of a standard reference reagent. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact with skin,eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, wash withcopious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azide yieldstoxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running water beforediscarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate <= 1 ug/106 cells <= 1 ug/106 cells <= 0.2 ug/106 cells |
| Application Info | Flow cytometry 7, Complement-dependent cytotoxicity 1, Immunoprecipitation 3, In vitro blocking of antigen presentation 5, Immunohistochemistry (frozen sections) 6 |
| Other Applications Data | Since applications vary, you should determine the optimum workingdilution of the product that is appropriate for your specific need. |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Each reagent is stable for the period shown on the bottle label ifstored as directed. |
| Reference | 1. Ozato, K., N. Mayer, and D.H. Sachs. 1980. J. Immunol. 124:533 2. Klein, J. 1986. Mutations in H- 2E loci. In: Natural History of the Major Histocompatibility Complex. John Wiley & Sons, New York, NY, pp216- 218 3. Neiss, U., and K. Reske. 1994. Int. Immunol. 6:61 4. Harton, J.A., W. Litaker, J.A. Frelinger, and G.A. Bishop. 1991. Immunogenetics 34:358 5. Roy, M., A. Aruffo, J. Ledbetter, P. Linsley, M. Kehry, and R. Noelle. 1995. Eur. J. Immunol. 25:596 6. Farr, A., P.C. DeRoos, S. Eastmaan, and A.Y. Rudensky. 1996. Eur. J. Immunol. 26:3185 7. Pennesi, G., M.J. Mattapallil, S.H. Sun, D. Avichezer, P.B. Silver, Z. Karabekian, C.S. David, P.A. Hargrave, J.H. McDowell, W.C. Smith, B Wiggert, L.A. Donoso, C. Chan, and R.R. Caspi. 2003. J. Clin. Invest. 111:1171. |
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| Gene Symbol | H2-EB1 |
| Gene Full Name | H-2 class II histocompatibility antigen, E-K alpha chain |
| Alias Symbols | I, Eb, Ia, Ia4, H2Eb, Ia-4, H-2Eb, H2-Eb_p |
| NCBI Gene Id | 14969 |
| Protein Name | H-2 class II histocompatibility antigen, I-E beta chain |
| Uniprot ID | P18469 |
