Mouse IFN alpha 1a Protein, His tag (Animal-Free)
Référence PRP1161-1000
Conditionnement : 1mg
Marque : Abbkine
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Specification
| Product name | Mouse IFN alpha 1a Protein, His tag (Animal-Free) |
| Sequence | Amino acid sequence derived from Mouse IFN alpha 1a (Cys24-Lys189) (P01572) was expressed with 6×His tag at the N-terminus |
| Applications | His tag |
| Applications notes | Mouse |
| Activity | Measure by its ability to protect L929 cells infected with encephalomyocarditis (EMC) virus.The ED₅₀ for this effect is <10 pg/mL. The specific activity of recombinant mouse IFN alpha 1a is > 4 x 10⁷ IU/mg. |
| Protein length | The recombinant Mouse IFN alpha 1a Protein consists of 166 amino acids and predicts a molecular mass of 19.93 kDa |
| Preparation method | E. coli |
| Purity | > 98% as determined by SDS-PAGE. |
| Alternative | Ifa, Ifa8 |
Product Properties
| Formulation | The protein was lyophilized from sterile PBS, pH 7.4. If you have any concerns or special requirements, please confirm with us. |
| Features & Benefits | Endotoxin: <0.1 EU per 1 μg of the protein by the LAL method. |
| Molecular weight | 19.93 kDa |
| Usage notes | Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant protein to a concentration of 0.1-1 mg/mL using the buffer we provided, and keep at room temperature for at least 20 min to fully dissolve. Please avoid vortex vigorously |
| Storage instructions | Lyophilized protein product should be stored desiccated below -18°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -18°C. For long term storage it is recommended to add a carrier protein (5% HSA , 10%FBS or 0.1%BSA). Please prevent freeze-thaw cycles |
| Shipping | The product is shipped with blue ice. |
| Precautions | The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product. |
Additional Information
| Background | Interferon Alpha 1a (IFN alpha 1a) is a leukocyte interferon,which is a variant of Interferon-alpha. IFN-alpha 1a is a 18.4 kDa protein containing 166 amino acid residues. It could bind the interferon receptors that activates the signal transduction of immune responses through the Jak/STAT pathway in leukocytes and lymphoblastoid cells. |
| Alternative | Ifa, Ifa8 |
| Accession | P01572 |
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