Mouse IgG3 (Gamma 3 chain) Antibody
Référence orb347529-1mg
Conditionnement : 1mg
Marque : Biorbyt
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
Description
Research Area
Immunology & Inflammation
Images & Validation
−
| Tested Applications | DOT, ELISA, IHC, WB |
|---|---|
| Dilution Range | ELISA: 1:10,000 - 1:50,000, IHC: 1:1,000 - 1:5,000, WB: 1:1,000 - 1:5,000 |
| Reactivity | Mouse |
| Application Notes |
Key Properties
−| Antibody Type | Secondary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Mouse IgG3 heavy chain |
| Purity | Anti-MOUSE IgG3 (Gamma 3 chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG3 coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Mouse Serum, Mouse IgG and Mouse IgG3. Specificity was confirmed by ELISA. For IgG1, IgG2a and IgG2b reactivity was less than 5% against target (IgG3, IgG). |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
|---|---|
| Form/Appearance | Liquid (sterile filtered) |
| Buffer/Preservatives | Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 |
| Concentration | 1.2 mg/mL |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Rabbit Anti-Mouse IgG3 (Gamma 3 chain) Antibody, Rabbit Anti Mouse IgG3 Antibody
−
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
DOT
Dot Blot