Orb347564-1mg | Mouse (lambda chain) Antibody Alkaline Phosphatase

Mouse (lambda chain) Antibody Alkaline Phosphatase Conjugated

SKU: orb347564

Mouse lambda (lambda chain) antibody (Alkaline Phosphatase)

Signal Transduction

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Tested Applications	ELISA, IHC, WB
Dilution Range	ELISA: 1:2,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
Reactivity	Mouse
Application Notes	Anti-Mouse λ Alkaline Phosphatase Conjugated Antibody has been tested by ELISA and is assayed against 1.0 ug of Mouse IgG in a standard capture ELISA using pNPP p-nitrophenyl phosphate as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:2,000 of the reconstitution concentration is suggested for this product.

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Antibody Type	Secondary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	IgG
Immunogen	Mouse lambda light chain
Purity	This product was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), and anti-Rabbit Serum. Solid phase adsorption(s) against a panel of Mouse IgG kappa, IgM kappa and IgA kappa proteins was performed. Specificity was confirmed by ELISA.. Specificity was confirmed by ELISA at less than 1% cross reactivity against other mouse or human heavy or light chain isotypes.
Conjugation	AP

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Storage	Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
Form/Appearance	Liquid (sterile filtered)
Buffer/Preservatives	Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.05 M Tris Chloride, 0.15M Sodium Chloride, 0.001M Magnesium Chloride, 0.0001M Zinc Chloride, 50% (v/v) Glycerol; pH 8.0
Concentration	1.0 mg/mL
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

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Rabbit Anti-Mouse λ (lambda chain) alkaline phosphatase Conjugated Antibody, Rabbit Anti-Mouse lambda Antibody alk phos conjugation

Western Blot (IB, immunoblot)

IHC

Immunohistochemistry

ELISA

Enzyme-linked Immunosorbent Assay (EIA)