Mouse Mecp2 (Methyl-CpG-binding protein 2) ELISA Kit
Référence MBS7606721-96
Conditionnement : 96-Strip-Wells
Marque : MyBiosource
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Mecp2 (Methyl-Cpg-Binding Protein 2) (MECP2) ELISA Kit for Mouse
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS7606721Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and… More details
Product Overview
Online Datasheet in PDFProduct Name
Mecp2 (Methyl-CpG-binding protein 2), ELISA Kit
Full Product Name
Mouse Mecp2 (Methyl-CpG-binding protein 2) ELISA Kit
Product Gene Name
Mecp2 elisa kit
Species Reactivity
Mouse
Assay Type
Sandwich
Samples
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
78.125-5000pg/ml
Sensitivity
46.875pg/ml
Specificity
This assay has high sensitivity and excellent specificity for detection of Mecp2. No significant cross-reactivity or interference between Mecp2 and analogues was observed.
Preparation and Storage
Store entire kit at 2-8C for short-term. For longer-term, please store the microplate & standard at -20C, while the remaining reagents can be stored at 2-8C
Other Notes
Small volumes of Mecp2 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Mecp2 purchase
MBS7606721 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mecp2 (Methyl-CpG-binding protein 2) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing Mecp2. The ELISA analytical biochemical technique of the MBS7606721 kit is based on Mecp2 antibody-Mecp2 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect Mecp2 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, Mecp2. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for Mecp2. It may not necessarily be applicable to this product.
NCBI Official Full Name
Methyl-CpG-binding protein 2
NCBI Official Synonym Full Names
methyl-CpG binding protein 2
NCBI Official Symbol
MECP2
NCBI Official Synonym Symbols
RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13
UniProt Protein Name
Methyl-CpG-binding protein 2
Protein Family
Methyl-CpG-binding protein
UniProt Gene Name
MECP2
UniProt Synonym Gene Names
MeCp-2 protein; MeCp2
NCBI Protein Information
methyl-CpG-binding protein 2
NCBI Summary for Mecp2
DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). Each of these proteins, with the exception of MBD3, is capable of binding specifically to methylated DNA. MECP2, MBD1 and MBD2 can also repress transcription from methylated gene promoters. In contrast to other MBD family members, MECP2 is X-linked and subject to X inactivation. MECP2 is dispensible in stem cells, but is essential for embryonic development. MECP2 gene mutations are the cause of most cases of Rett syndrome, a progressive neurologic developmental disorder and one of the most common causes of mental retardation in females. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015]
Show more UniProt Comments for Mecp2
Chromosomal protein that binds to methylated DNA. It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. Mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. Binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC).
Show more Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with Mecp2 elisa kit
| Reference Product | PubMed Publications |
|---|---|
| BDNF elisa kit | >159 publications with Mecp2 and BDNF |
| DNMT1 elisa kit | >122 publications with Mecp2 and DNMT1 |
| CDKL5 elisa kit | >115 publications with Mecp2 and CDKL5 |
| HDAC1 elisa kit | >54 publications with Mecp2 and HDAC1 |
| SIN3A elisa kit | >30 publications with Mecp2 and SIN3A |
| ATRX elisa kit | >24 publications with Mecp2 and ATRX |
| CREB1 elisa kit | >10 publications with Mecp2 and CREB1 |
| RCOR1 elisa kit | >4 publications with Mecp2 and RCOR1 |
| CXXC1 elisa kit | >1 publications with Mecp2 and CXXC1 |
Pathways associated with Mecp2 elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Cytosine Methylation Pathway antibodies | Cytosine Methylation Pathway Diagram |
| Ectoderm Differentiation Pathway antibodies | Ectoderm Differentiation Pathway Diagram |
| Lung Fibrosis Pathway antibodies | Lung Fibrosis Pathway Diagram |
| MECP2 And Associated Rett Syndrome Pathway antibodies | MECP2 And Associated Rett Syndrome Pathway Diagram |
| Sudden Infant Death Syndrome (SIDS) Susceptibility Pathways antibodies | Sudden Infant Death Syndrome (SIDS) Susceptibility Pathways Diagram |
Diseases associated with Mecp2 elisa kit
| Disease Name | Pubmed Publications |
|---|---|
| Rett Syndrome Antibodies | >1519 publications with Mecp2 and Rett Syndrome |
| Intellectual Disability Antibodies | >1285 publications with Mecp2 and Intellectual Disability |
| Neurodevelopmental Disorders Antibodies | >476 publications with Mecp2 and Neurodevelopmental Disorders |
| Seizures Antibodies | >182 publications with Mecp2 and Seizures |
| Autistic Disorder Antibodies | >164 publications with Mecp2 and Autistic Disorder |
| Child Development Disorders, Pervasive Antibodies | >124 publications with Mecp2 and Child Development Disorders, Pervasive |
| Learning Disorders Antibodies | >60 publications with Mecp2 and Learning Disorders |
| Developmental Disabilities Antibodies | >59 publications with Mecp2 and Developmental Disabilities |
| Inflammation Antibodies | >47 publications with Mecp2 and Inflammation |
| Cognition Disorders Antibodies | >44 publications with Mecp2 and Cognition Disorders |
Organs/Tissues associated with Mecp2 elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >997 publications with Mecp2 and Brain |
| Nerve Antibodies | >178 publications with Mecp2 and Nerve |
| Muscle Antibodies | >73 publications with Mecp2 and Muscle |
| Liver Antibodies | >60 publications with Mecp2 and Liver |
| Heart Antibodies | >56 publications with Mecp2 and Heart |
| Eye Antibodies | >40 publications with Mecp2 and Eye |
| Embryonic Tissue Antibodies | >39 publications with Mecp2 and Embryonic Tissue |
| Lung Antibodies | >36 publications with Mecp2 and Lung |
| Bone Antibodies | >35 publications with Mecp2 and Bone |
| Prostate Antibodies | >22 publications with Mecp2 and Prostate |