Mouse MHC Class II Antibody : Biotin
Référence OASB00665
Conditionnement : 0.5mg
Marque : Aviva Systems Biology
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Mouse MHC Class II Antibody : Biotin (OASB00665)
| Datasheets/Manuals | Printable datasheet for Mouse MHC Class II Antibody : Biotin (OASB00665) |
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| Tested Species Reactivity | Mouse |
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| Predicted Species Reactivity | Mouse |
| Clonality | Monoclonal |
| Clone | NIMR-4 |
| Isotype | IgG2b |
| Host | Rat |
| Conjugation | Biotin |
| Application | FC, IHC-F, ICC |
| Additional Information | Description: The monoclonal antibody NIMR-4 reacts specifically with a non-polymorphic I-A-encoded epitope on MHC Class II antigens. Class II antigens are predominantly expressed on antigen-presenting cells including B lymphocytes, macrophages, dendritic cells, and certain epithelial cells. |
| Reconstitution and Storage | Store at 2-8C |
| Concentration | 0.5 mg/mL |
| Specificity | MHC Class II |
| Characterization | To ensure lot- to- lot consistency, each batch of product is tested by flow cytometry to conform tocharacteristics of a standard reference reagent. Representative data are included in this product insert. |
| Warning | Reagents contain sodium azide. Sodium azide is very toxic if ingested or inhaled. Avoid contact withskin, eyes, or clothing. Wear eye or face protection when handling. If skin or eye contact occurs, washwith copious amounts of water. If ingested or inhaled, contact a physician immediately. Sodium azideyields toxic hydrazoic acid under acidic conditions. Dilute azide- containing compounds in running waterbefore discarding to avoid accumulation of potentially explosive deposits in lead or copper plumbing. |
| Dilution | Flow Cytometry: Purified antibody Fluorescein conjugate Biotin conjugate R-phycoerythrin conjugate Spectral Red conjugate <=1 ug/106 cells <=1 ug/106 cells <=1 ug/106 cells <=0.2 ug/106 cells <=0.2 ug/106 cells |
| Application Info | Flow cytometry 1,2 |
| Other Applications Data | Since applications vary, each investigator should determine the optimum working dilutions of the product that is appropriate for their specificneeds. |
| Other Applications Image 1 Data | Amount Used: ≤1 Ug/106 cells BALB/c spleen cells were stained with Rat Anti-Mouse MHC II-FITC, following which small lymphocytes were gated and analyzed on a FACScan flow cytometer (BDIS, San Jose, CA). |
| Storage | - The purified (UNLB) antibody is supplied as 0.5 mg of purified immunoglobulin in 1.0 mL of 100 mMborate buffered saline, pH 8.2. No preservatives or amine- containing buffer salts added. Store at 2- 8 C - The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL of PBS/NaN3. Store at 2- 8 C - The R- phycoerythrin (R- PE) conjugate is supplied as 0.1 mg in 1.0 mL or 0.2 mg in 2.0 mL ofPBS/NaN3 and a stabilizing agent. Store at 2- 8 C. Do not freeze! - The Spectral Red (SPRD) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizingagent. Store at 2- 8 C. Do not freeze! - The low endotoxin, azide- free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mLof PBS. Aliquot and store at or below - 20 C - Protect conjugated forms from light. Aliquot and freeze the low endotoxin, azide- free product at - 20 Cimmediately upon receipt. Each reagent is stable for the period shown on the bottle label if stored asdirected. |
| Reference | 1. R.M.E. Parkhouse. 1996. Personal communication 2. Agger, R., M.S. Petersen, H.E. Toldbod, S. Holtz, F. Dagnaes- Hansen, B.W. Johnsen, L. Bolund, and M. Hokland. 2000. Scand. J. .. . … Immunol. 52:138. |
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| Gene Symbol | H2-AA |
| Gene Full Name | HLA class II histocompatibility antigen gamma chain |
| Alias Symbols | A, I, I-, Ia, H-2, Ia1, H2Aa, Ia-1, H-2Aa, Aalpha, IAalpha, I-Aalpha |
| NCBI Gene Id | 14960 |
| Protein Name | histocompatibility 2, class II antigen A, alpha |
| Uniprot ID | P01910, P04227, P04228, P14434, P14435, P14436, P14437, P14438, P23150 |
