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Mouse MIP-2 ELISA Kit
Mouse MIP-2 ELISA Kit
RayBio® Mouse MIP-2 ELISA Kit for cell culture supernatants, plasma, and serum samples.
Lead time: Typically ships within 1-2 business days. No Friday shipments.
Contact Us for Bulk Request
Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Mouse |
| Sensitivity | 10 pg/ml Need more sensitivity? Check out the new NexaTag® kit for this target. Still not enough? Then your answer is our Ultrasensitive Biomarker Testing Service powered by Simoa™ technology. |
| Detection Range | 10 pg/ml - 2000 pg/ml |
| Specificity | This ELISA kit shows no cross-reactivity with any of the cytokines tested: Mouse CD30, L CD30, T CD40, CRG-2, CTACK, CXCL16, Eotaxin , Eotaxin-2, Fas Ligand, Fractalkine, GCSF, GM-CFS, IFN- gamma, IGFBP-3, IGFBP-5, IGFBP-6, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-3 Rb, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12 p40/p70, IL-12 p70, IL-13, IL-17, KC, Leptin R, LEPTIN(OB), LIX, L-Selectin, Lymphotactin, MCP-1, MCP-5, M-CSF, MIG, MIP-1 alpha, MIP-1 gamma, MIP-3 beta, MIP-3 alpha, PF-4, P-Selectin, RANTES, SCF, SDF-1 alpha, TARC, TCA-3, TECK, TIMP-1, TNF-alpha, TNF RI, TNF RII, TPO, VCAM-1, VEGF. |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum |
| Recommended Dilution (Serum/Plasma) | 2 fold |
| Accession Number | P10889 |
| Gene Id | 20310 |
| Gene Symbols | CXCL2, MIP-2, MIP2, SCYB2 |
| Protein Name / Synonyms | C-X-C motif chemokine 2 (Macrophage inflammatory protein 2) (MIP2) |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Sandwich-based |
| Method Of Detection | Colorimetric |
| Research Area | Inflammation |
| Shipping Type | Blue ice |
| Storage | ≤-20°C |
Related Products
NexaTag® Mouse MIP-2 qIPCR ELISA Kit
As low as $749.00
RayPlex® Mouse MIP-2 Bead Assay
As low as $240.00
Product Features
Additional Notes
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Standard Curves
Spiking & Recovery Results
Linearity Results
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 µl volumes
- Adjustable 1-25 µl pipettes for reagent preparation
- 100 µl and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
Protocol Outline
- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 µl of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4°C.
- Add 100 µl of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 µl of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 µl of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl of Stop Solution to each well.
- Read at 450 nm immediately.
Storage/Stability
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Customize This Mouse MIP-2 ELISA
We manufacture our ELISAs in-house, so we can build a custom version for your study.
- Post-translational modifications (PTMs): Detect modified CXCL2 (CINC-3 / MIP2), including phosphorylation, acetylation, S-nitrosylation, S-acylation, oxidation, lactylation, sumoylation, ubiquitination, nitration, carbamylation, and more
- Sample types: Validation in your matrix using samples you provide, such as CSF, urine, saliva, lysates, or exosomes
- Also available: More sensitive platforms such as Simoa® and NexaTag®, multiplex panels, bulk lots, and other species when suitable reagents exist
Feasibility varies by target and modification, and we’ll tell you upfront what’s achievable.
Learn more about custom ELISA development
documents
citations
A mosquito salivary protein-driven influx of myeloid cells facilitates flavivirus transmission
- Species:
- Mouse
- Sample Type:
- Cell Culture Supernatant (footpad lysate supernatants)
- Year:
- 2024
- Journal Name:
- EMBO Journal
- PubMed ID:
- 38378891
Granzyme B inhibition reduces disease severity in autoimmune blistering diseases
- Species:
- Mouse
- Sample Type:
- Other / Unspecified (mouse lesional skins)
- Year:
- 2021
- Journal Name:
- Nature Communications
- PubMed ID:
- 33436591
Chemotaxis-driven delivery of nano-pathogenoids for complete eradication of tumors post-phototherapy
- Species:
- Mouse
- Sample Type:
- Tissue Lysate
- Year:
- 2020
- Journal Name:
- Nature Communications
- PubMed ID:
- 32111847
PMN-MDSCs modulated by CCL20 from cancer cells promoted breast cancer cell stemness through CXCL2-CXCR2 pathway
- Species:
- Mouse
- Sample Type:
- 4T1 cell, Py8119 cell
- Year:
- 2023
- Journal Name:
- Signal Transduction and Targeted Therapy
MicroRNA let-7 Modulates the Immune Response to Mycobacterium tuberculosis Infection via Control of A20, an Inhibitor of the NF-kB Pathway
- Species:
- Mouse
- Sample Type:
- Conditioned Media (A20-/- macrophages)
- Year:
- 2015
- Journal Name:
- Cell Host and Microbe
- PubMed ID:
- 25683052
Neutrophil extracellular traps amplify neutrophil recruitment and inflammation in neutrophilic asthma by stimulating the airway epithelial cells to activate the TLR4/ NF-κB pathway and secrete chemokines
- Species:
- Mouse
- Sample Type:
- Bronchoalveolar Lavage (BALF supernatant)
- Year:
- 2020
- Journal Name:
- Aging
- PubMed ID:
- 32756014
Loss of SPARC in bladder cancer enhances carcinogenesis and progression
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (bladder)
- Year:
- 2013
- Journal Name:
- Journal of Clinical Investigation
An infection-enhanced oncolytic adenovirus secreting H. pylori neutrophil-activating protein with therapeutic effects on neuroendocrine tumors.
- Species:
- Mouse
- Sample Type:
- Plasma (Neuroendocrine Tumor model)
- Year:
- 2013
- Journal Name:
- Molecular Therapy
Effects of globin digest and its active ingredient Trp-Thr-Gln-Arg on galactosamine/lipopolysaccharide-induced liver injury in ICR mice
- Species:
- Mouse
- Sample Type:
- Conditioned Media (LPS stimulated RAW macrophages); Serum (Liver injury model, ICR mice)
- Year:
- 2012
- Journal Name:
- Life Sciences
- PubMed ID:
- 22154906
Conditional overexpression of receptors for advanced glycation end-products in the adult murine lung causes airspace enlargement and induces inflammation.
- Species:
- Mouse
- Sample Type:
- BAL Fluid (RAGE in mouse lung causes airspace enlargement and Induces inflammation)
- Year:
- 2013
- Journal Name:
- American Journal of Respiratory Cell and Molecular Biology
- PubMed ID:
- 23526218
