Mouse Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) ELISA Kit
Référence MBS2090467-48
Conditionnement : 48-Strip-Wells
Marque : MyBiosource
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Mitochondrial Open Reading Frame of the 12s Rrna-C (MOTS-c) ELISA Kit for Mouse
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2090467SPECIAL PROMOTION: Get FREE Starbucks gift card ( for 48-Wells-Kit or for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment
Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. An antibody specific to MOTS-c has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled MOTS-c and unlabeled… More details
Product Overview
Online Datasheet in PDFProduct Name
Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c), ELISA Kit
Full Product Name
Mouse Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) ELISA Kit
Product Gene Name
MOTS-c elisa kit
Species Reactivity
Mouse
Assay Type
Quantitative Competitive
Samples
Mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
246.9-20,000pg/mL
Sensitivity
< 95.1pg/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of MOTS-c. No significant cross-reactivity or interference between MOTS-c and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level MOTS-c were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level MOTS-c were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Categories/Family for MOTS-c elisa kit
Developmental science
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Product References and Citations for MOTS-c elisa kit
Mitohormesis in Hypothalamic POMC Neurons Mediates Regular Exercise-Induced High-Turnover Metabolism; Cell Metab; 2021 Feb 2;33(2):334-349.e6.; PMID: 33535098; Gil Myoung Kang; Asan Institute for Life Science, University of Ulsan College of Medicine, Seoul 05505, Korea.
Other Notes
Small volumes of MOTS-c elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for MOTS-c purchase
MBS2090467 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Mitochondrial Open Reading Frame Of The 12S rRNA-c (MOTS-c) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing MOTS-c. The ELISA analytical biochemical technique of the MBS2090467 kit is based on MOTS-c antibody-MOTS-c antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect MOTS-c antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, MOTS-c. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
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