Quantibody® Mouse Th17 Array 1 Kit. Detects 18 Mouse Th1, Th2, and Th17 Cytokines. Suitable for all liquid sample types.
Mouse Th1/Th2/Th17 Array Q1
Mouse Th1/Th2/Th17 Array Q1
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Product Description
Specifications
| Size | 8 Sample Kit, 22 Sample Kit, 50 Sample Kit |
| Species | Mouse |
| Compatible Sample Types | Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Cell Lysates |
| Quantitative/Semi-Quantitative | Quantitative |
| Number of Targets Detected | 18 |
| Solid Support | Glass Slide |
| Design Principle | Sandwich-based |
| Method Of Detection | Fluorescence Laser Scanner |
| Research Area | T Helper Cells, Adaptive Immunity |
| Shipping Type | Blue ice |
| Storage | -20°C |
Product Features
- More cost-effective than traditional ELISA
- High specificity and system reproducibility
- Suitable for diverse sample types
- Low sample volume requirement: 50 µL or less
- Get results same day (6-hour processing time)
- Well-suited for high throughput assays
- Q Analyzer software provides one-step computation
Target Names
| Scroll over each target protein for more information | ||||
|---|---|---|---|---|
| IFN-gamma
| IL-1 beta (IL-1 F2)
| IL-10
| IL-12 p70
| IL-13
|
| IL-17A
| IL-17F
| IL-2
| IL-21
| IL-22
|
| IL-23
| IL-28A (IFN-lambda 2)
| IL-4
| IL-5
| IL-6
|
| MIP-3 alpha (CCL20)
| TGF beta 1
| TNF alpha
| ||
Application Notes
Suggested Application
Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range
Kit Components
- Mouse Th1/Th2/Th17 Array Q2 Slide(s)
- Blocking Buffer
- Wash Buffer 1
- Wash Buffer 2
- Lyophilized Standard Mix
- Biotinylated Detection Antibody Cocktail
- Streptavidin-Conjugated Fluor
- Slide Washer/Dryer
- Adhesive Plastic Strips
- Manual
Other Materials Required
- Distilled or deionized water
- Small plastic boxes or containers
- Pipettors, pipette tips and other common lab consumables
- Orbital shaker or oscillating rocker
- Aluminum foil
- Gene microarray scanner or similar laser fluorescence scanner
View Compatible Laser Scanners
Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More
Protocol Outline
- Dry the glass slide
- Prepare Standards
- Block array surface
- Incubate with Samples and Standards
- Incubate with Biotinylated Detection Antibody Cocktail
- Incubate with Streptavidin-Conjugated Fluor
- Disassemble the glass slide
- Scan with a gene microarray laser scanner
- Perform densitometry and analysis
Storage/Stability
For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.
documents
GAL (GAL-QAM-TH17-1.gal)
Excel (QAM-TH17-1_Analysis_Tool.xls)
citations
Preeclampsia promotes autism in offspring via maternal inflammation and fetal NFκB signaling
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2023
- Journal Name:
- Life science alliance
Effects of Probiotics on Colitis-Induced Exacerbation of Alzheimer’s Disease in AppNL-G-F Mice
- Species:
- Mouse
- Sample Type:
- brain
- Year:
- 2023
- Journal Name:
- International Journal of Molecular Sciences
A Comparison of the Impact of Restrictive Diets on the Gastrointestinal Tract of Mice
- Species:
- Mouse
- Sample Type:
- small intestine mucosa
- Year:
- 2022
- Journal Name:
- Nutrients
Chronic alcohol-induced dysbiosis of the gut microbiota and gut metabolites impairs sperm quality in mice
- Species:
- Mouse
- Sample Type:
- Serum, testis
- Year:
- 2022
- Journal Name:
- Frontiers in microbiology
Potential Chemoprevention of Paeoniflorin in Colitis-Associated Colorectal Cancer by Network Pharmacology, Molecular Docking, and In Vivo Experiment
- Species:
- Mouse
- Sample Type:
- Serum
- Year:
- 2022
- Journal Name:
- Chemistry & Biodiversity
Notch signaling regulates antigen sensitivity of naive CD4+ T cells by tuning co-stimulation
- Species:
- Mouse
- Sample Type:
- Conditioned Media (5cc7 cells in Notch signaling and co-stimulation model)
- Year:
- 2015
- Journal Name:
- Immunity
- PubMed ID:
- 25607460
Periodontal pathogens invade gingiva and aortic adventitia and elicit inflammasome activation in v6 integrin-deficient mice
- Species:
- Human
- Sample Type:
- Conditioned Media (A375 ERK2 treated cells with or without ddoxycycline); Conditioned Media (Splenic cultures from gingival bacterial infected mice)
- Year:
- 2015
- Journal Name:
- Infection and Immunity
- PubMed ID:
- 26371120
A composite demineralized bone matrix e Self assembling peptide scaffold for enhancing cell and growth factor activity in bone marrow
- Species:
- Mouse
- Sample Type:
- Tissue Lysate (Deminarlized Bone Matrix)
- Year:
- 2014
- Journal Name:
- Biomaterials
Impaired B cell Inhibition by Lupus Bone Marrow Mesenchymal Stem Cells is Caused by Reduced CCL2 Expression
- Species:
- Mouse
- Sample Type:
- Conditioned media (Bone marrow mesenchymal stem cells)
- Year:
- 2014
- Journal Name:
- The Journal of Immunology
A novel chronic stress-induced shift in the Th1 to Th2 response promotes colon cancer growth.
- Species:
- Mouse
- Sample Type:
- Serum (stressed mice with colon cancer model)
- Year:
- 2013
- Journal Name:
- Biochemical and Biophysical Research Communications
- PubMed ID:
- 24036270