Mouse Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) ELISA Kit
Référence MBS2022044-96
Conditionnement : 96-Strip-Wells
Marque : MyBiosource
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Wingless Type MMTV Integration Site Family, Member 3a (WNT3A) ELISA Kit for Mouse
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2022044SPECIAL PROMOTION: Get FREE Starbucks gift card ( for 48-Wells-Kit or for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of WNT3A in mouse serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.Principle of the Assay: The microplate… More details
Product Overview
Product Name
Wingless Type MMTV Integration Site Family, Member 3A (WNT3A), ELISA Kit
Full Product Name
Mouse Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) ELISA Kit
Product Gene Name
WNT3A elisa kit
Species Reactivity
Mouse
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates, Cell culture supernates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.059ng/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of WNT3A. No significant cross-reactivity or interference between WNT3A and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level WNT3A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level WNT3A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Molecular Weight
42,905 Da
OMIM
606359
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Categories/Family for WNT3A elisa kit
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Other Notes
Small volumes of WNT3A elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for WNT3A purchase
MBS2022044 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Wingless Type MMTV Integration Site Family, Member 3A (WNT3A) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing WNT3A. The ELISA analytical biochemical technique of the MBS2022044 kit is based on WNT3A antibody-WNT3A antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect WNT3A antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, WNT3A. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for WNT3A. It may not necessarily be applicable to this product.
NCBI Official Full Name
wingless-type MMTV integration site family, member 3A
NCBI Official Synonym Full Names
wingless-type MMTV integration site family, member 3A
NCBI Official Symbol
WNT3A
UniProt Protein Name
Protein Wnt-3a
UniProt Gene Name
WNT3A
UniProt Entry Name
WNT3A_HUMAN
NCBI Protein Information
protein Wnt-3a
NCBI Summary for WNT3A
The WNT gene family consists of structurally related genes which encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It encodes a protein which shows 96% amino acid identity to mouse Wnt3A protein, and 84% to human WNT3 protein, another WNT gene product. This gene is clustered with WNT14 gene, another family member, in chromosome 1q42 region. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for WNT3A
WNT3A: Ligand for members of the frizzled family of seven transmembrane receptors. Wnt-3 and Wnt-3a play distinct roles in cell-cell signaling during morphogenesis of the developing neural tube. Interacts with PORCN. Interacts with APCDD1 and WLS. Component of the Wnt-Fzd-LRP5-LRP6 signaling complex that contains a WNT protein, a FZD protein and LRP5 or LRP6. Interacts directly in the complex with LRP6. Moderately expressed in placenta and at low levels in adult lung, spleen, and prostate. Belongs to the Wnt family.
Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1q42
Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; endoplasmic reticulum lumen; early endosome membrane; Golgi lumen; extracellular region; plasma membrane
Molecular Function: protein domain specific binding; protein binding; frizzled binding; transcription coactivator activity; receptor agonist activity; frizzled-2 binding
Biological Process: positive regulation of mesodermal cell fate specification; axon guidance; extracellular matrix organization and biogenesis; positive regulation of protein binding; positive regulation of transcription, DNA-dependent; cell proliferation in forebrain; positive regulation of receptor internalization; positive regulation of caspase activity; positive regulation of collateral sprouting in the absence of injury; Wnt receptor signaling pathway through beta-catenin; palate development; negative regulation of axon extension involved in axon guidance; Wnt receptor signaling pathway in forebrain neuroblast division; negative regulation of neurogenesis; neuron differentiation; mammary gland development; positive regulation of cell proliferation; positive regulation of B cell proliferation; hemopoiesis; heart looping; dorsoventral neural tube patterning; inner ear morphogenesis; in utero embryonic development; hippocampus development; negative regulation of fat cell differentiation; positive regulation of peptidyl-serine phosphorylation; osteoblast differentiation; paraxial mesodermal cell fate commitment; signalosome assembly; spinal cord association neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation
Show more Protein type: Secreted; Secreted, signal peptide
Chromosomal Location of Human Ortholog: 1q42
Cellular Component: proteinaceous extracellular matrix; extracellular space; cell surface; endoplasmic reticulum lumen; early endosome membrane; Golgi lumen; extracellular region; plasma membrane
Molecular Function: protein domain specific binding; protein binding; frizzled binding; transcription coactivator activity; receptor agonist activity; frizzled-2 binding
Biological Process: positive regulation of mesodermal cell fate specification; axon guidance; extracellular matrix organization and biogenesis; positive regulation of protein binding; positive regulation of transcription, DNA-dependent; cell proliferation in forebrain; positive regulation of receptor internalization; positive regulation of caspase activity; positive regulation of collateral sprouting in the absence of injury; Wnt receptor signaling pathway through beta-catenin; palate development; negative regulation of axon extension involved in axon guidance; Wnt receptor signaling pathway in forebrain neuroblast division; negative regulation of neurogenesis; neuron differentiation; mammary gland development; positive regulation of cell proliferation; positive regulation of B cell proliferation; hemopoiesis; heart looping; dorsoventral neural tube patterning; inner ear morphogenesis; in utero embryonic development; hippocampus development; negative regulation of fat cell differentiation; positive regulation of peptidyl-serine phosphorylation; osteoblast differentiation; paraxial mesodermal cell fate commitment; signalosome assembly; spinal cord association neuron differentiation; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; positive regulation of protein amino acid phosphorylation
Research Articles on WNT3A
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with WNT3A elisa kit
| Reference Product | PubMed Publications |
|---|---|
| CTNNB1 elisa kit | >731 publications with WNT3A and CTNNB1 |
| DKK1 elisa kit | >128 publications with WNT3A and DKK1 |
| LRP6 elisa kit | >100 publications with WNT3A and LRP6 |
| FZD1 elisa kit | >20 publications with WNT3A and FZD1 |
| DVL2 elisa kit | >17 publications with WNT3A and DVL2 |
| AXIN1 elisa kit | >14 publications with WNT3A and AXIN1 |
| DVL3 elisa kit | >12 publications with WNT3A and DVL3 |
| FZD8 elisa kit | >8 publications with WNT3A and FZD8 |
| WIF1 elisa kit | >7 publications with WNT3A and WIF1 |
| DVL1 elisa kit | >5 publications with WNT3A and DVL1 |
Pathways associated with WNT3A elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Basal Cell Carcinoma Pathway antibodies | Basal Cell Carcinoma Pathway Diagram |
| Basal Cell Carcinoma Pathway antibodies | Basal Cell Carcinoma Pathway Diagram |
| Canonical Wnt Signaling Pathway antibodies | Canonical Wnt Signaling Pathway Diagram |
| Cardiac Progenitor Differentiation Pathway antibodies | Cardiac Progenitor Differentiation Pathway Diagram |
| Class B/2 (Secretin Family Receptors) Pathway antibodies | Class B/2 (Secretin Family Receptors) Pathway Diagram |
| DNA Damage Response (only ATM Dependent) Pathway antibodies | DNA Damage Response (only ATM Dependent) Pathway Diagram |
| Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway antibodies | Defective ACTH Causes Obesity And Pro-opiomelanocortinin Deficiency (POMCD) Pathway Diagram |
| Disease Pathway antibodies | Disease Pathway Diagram |
| GPCR Ligand Binding Pathway antibodies | GPCR Ligand Binding Pathway Diagram |
| HTLV-I Infection Pathway antibodies | HTLV-I Infection Pathway Diagram |
Diseases associated with WNT3A elisa kit
| Disease Name | Pubmed Publications |
|---|---|
| Breast Neoplasms Antibodies | >36 publications with WNT3A and Breast Neoplasms |
| Necrosis Antibodies | >28 publications with WNT3A and Necrosis |
| Fibrosis Antibodies | >27 publications with WNT3A and Fibrosis |
| Inflammation Antibodies | >27 publications with WNT3A and Inflammation |
| Congenital Abnormalities Antibodies | >25 publications with WNT3A and Congenital Abnormalities |
| Liver Diseases Antibodies | >22 publications with WNT3A and Liver Diseases |
| Liver Neoplasms Antibodies | >17 publications with WNT3A and Liver Neoplasms |
| Brain Diseases Antibodies | >17 publications with WNT3A and Brain Diseases |
| Lung Diseases Antibodies | >16 publications with WNT3A and Lung Diseases |
| Neoplasms, Experimental Antibodies | >14 publications with WNT3A and Neoplasms, Experimental |
Organs/Tissues associated with WNT3A elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Connective Tissue Antibodies | >55 publications with WNT3A and Connective Tissue |
| Lung Antibodies | >39 publications with WNT3A and Lung |
| Placenta Antibodies | >11 publications with WNT3A and Placenta |
| Testis Antibodies | >6 publications with WNT3A and Testis |
| Trachea Antibodies | >2 publications with WNT3A and Trachea |
| Uncharacterized Tissue Antibodies | >1 publications with WNT3A and Uncharacterized Tissue |
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