MS4A1 Antibody
Référence OAEE00718
Conditionnement : 100tests(T100)
Marque : Aviva Systems Biology
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
| Datasheets/Manuals | Printable datasheet for MS4A1 Antibody |
|---|
| Predicted Species Reactivity | Human|Non-human Primate |
|---|---|
| Product Format | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Clonality | Monoclonal |
| Clone | 2H7 |
| Isotype | IgG2b |
| Host | Mouse |
| Conjugation | PerCP |
| Application | FC |
| Reconstitution and Storage | 2°C to 8°C |
| Immunogen | Human tonsillar B cells |
| Purification | Purified by size-exclusion chromatography |
| Concentration | 10 ul / test |
| Specificity | The mouse monoclonal antibody 2H7 recognizes CD20 (B1, Bp35), a 33-37 kDa non-glycosylated membrane receptor with four transmembrane domains, expressed on pre-B lymphocytes, resting and activated B cells (not plasma cells), follicular dendritic cells, and at low levels on peripheral blood T lymphocytes. |
| Formulation | PerCP |
| Storage Buffer | The reagent is provided in phosphate buffered saline (PBS) containing 15 mM sodium azide and 0.2% (w/v) high-grade protease free Bovine Serum Albumin (BSA) as a stabilizing agent. |
| Reference | Polyak MJ, Ayer LM, Szczepek AJ, Deans JP: A cholesterol-dependent CD20 epitope detected by the FMC7 antibody. Leukemia. 2003 Jul;17(7):1384-9. Hayden-Ledbetter MS, Cerveny CG, Espling E, Brady WA, Grosmaire LS, Tan P, Bader R, Slater S, Nilsson CA, Barone DS, Simon A, Bradley C, Thompson PA, Wahl AF, Ledbetter JA: CD20-directed small modular immunopharmaceutical, TRU-015, depletes normal and malignant B cells. Clin Cancer Res. 2009 Apr 15;15(8):2739-46. Diehl SA, Schmidlin H, Nagasawa M, van Haren SD, Kwakkenbos MJ, Yasuda E, Beaumont T, Scheeren FA, Spits H: STAT3-mediated up-regulation of BLIMP1 Is coordinated with BCL6 down-regulation to control human plasma cell differentiation. J Immunol. 2008 Apr 1;180(7):4805-15. Deans JP, Robbins SM, Polyak MJ, Savage JA: Rapid redistribution of CD20 to a low density detergent-insoluble membrane compartment. J Biol Chem. 1998 Jan 2;273(1):344-8. Deans JP, Kalt L, Ledbetter JA, Schieven GL, Bolen JB, Johnson P: Association of 75/80-kDa phosphoproteins and the tyrosine kinases Lyn, Fyn, and Lck with the B cell molecule CD20. Evidence against involvement of the cytoplasmic regions of CD20. J Biol Chem. 1995 Sep 22;270(38):22632-8. Rose AL, Smith BE, Maloney DG: Glucocorticoids and rituximab in vitro: synergistic direct antiproliferative and apoptotic effects. Blood. 2002 Sep 1;100(5):1765-73. Polyak MJ, Deans JP: Alanine-170 and proline-172 are critical determinants for extracellular CD20 epitopes; heterogeneity in the fine specificity of CD20 monoclonal antibodies is defined by additional requirements imposed by both amino acid sequence and quaternary structure. Blood. 2002 May 1;99(9):3256-62. Chan HT, Hughes D, French RR, Tutt AL, Walshe CA, Teeling JL, Glennie MJ, Cragg MS: CD20-induced lymphoma cell death is independent of both caspases and its redistribution into triton X-100 insoluble membrane rafts. Cancer Res. 2003 Sep 1;63(17):5480-9. Leukocyte Typing V., Schlossman S. et al. (Eds.), Oxford University Press (1995). Leukocyte Typing IV., Knapp W. et al. (Eds.), Oxford University Press (1989). Leukocyte Typing III., McMichael M.J. et al. (Eds.), Oxford University Press (1987); p.611. Leukocyte Typing Human B Cell and B cell blast-associated Surface Molecules defined with Monoclonal Antibodies, Clerk et al., 1984, pg. 339-346. |
|---|---|
| Gene Symbol | MS4A1 |
| Gene Full Name | membrane spanning 4-domains A1 |
| Alias Symbols | B-lymphocyte antigen CD20, B-lymphocyte cell-surface antigen B1, B-lymphocyte surface antigen B1, B1, Bp35, CD20, CD20 antigen, CD20 receptor, CVID5, FMC7, LEU-16, leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, membrane-spanning 4-domains, subfamily A, member 1, S7. |
| NCBI Gene Id | 931 |
| Protein Name | B-lymphocyte antigen CD20 |
| Description of Target | This protein may be involved in the regulation of B-cell activation and proliferation. |
| Uniprot ID | P11836 |
| Protein Accession # | NP_068769.2; NP_690605.1 |
| Nucleotide Accession # | NM_021950.3; NM_152866.2 |
