Multi-species Synaptosomal Associated Protein 25kDa (SNAP25) ELISA Kit
Référence MBS2025154-96
Conditionnement : 96-Strip-Wells
Marque : MyBiosource
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Synaptosomal Associated Protein 25kDa (SNAP25) ELISA Kit for Human, Mouse, Rat, Rabbit & More
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2025154SPECIAL PROMOTION: Get FREE Starbucks gift card ( for 48-Wells-Kit or for 96-Wells-Kit. Maximum 5 gift cards/product). Gift card(s) will be included with shipment
Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of smoothened in rat tissue homogenates, cell lysates, cell culture supernates and other biological fluids.Principle of the Assay: The microplate provided in this… More details
Product Overview
Online Datasheet in PDFProduct Name
Synaptosomal Associated Protein 25kDa (SNAP25), ELISA Kit
Full Product Name
Multi-species Synaptosomal Associated Protein 25kDa (SNAP25) ELISA Kit
Product Gene Name
SNAP25 elisa kit
Product Synonym Names
Human/Mouse/Rat/Rabbit/Pig/Bovine/Horse/Chicken Synaptosomal Associated Protein 25kDa (SNAP25) (Sandwich ELISA) ELISA Kit; RIC4; SEC9; SNAP; SUP; Super protein; Resistance To Inhibitors Of Cholinesterase 4 Homolog
Species Reactivity
Human, Mouse, Rat, Rabbit, Pig, Bovine, Horse, Chicken
Assay Type
Double-antibody Sandwich
Samples
Tissue homogenates, Cell lysates and other biological fluids
Detection Range
0.156-10ng/mL
Sensitivity
0.059ng/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of smoothened. No significant cross-reactivity or interference between smoothened and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Inter-assay Precision
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level smoothened were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
Intra-assay Precision
Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level smoothened were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
Molecular Weight
23,336 Da
OMIM
600322
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Product Categories/Family for SNAP25 elisa kit
Neuroscience
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Other Notes
Small volumes of SNAP25 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for SNAP25 purchase
MBS2025154 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Synaptosomal Associated Protein 25kDa (SNAP25) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing SNAP25. The ELISA analytical biochemical technique of the MBS2025154 kit is based on SNAP25 antibody-SNAP25 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect SNAP25 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, SNAP25. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).
Additional Details
NCBI/Uniprot data below describe general gene information for SNAP25. It may not necessarily be applicable to this product.
NCBI Official Full Name
synaptosomal-associated protein 25 isoform SNAP25B
NCBI Official Synonym Full Names
synaptosomal-associated protein, 25kDa
NCBI Official Symbol
SNAP25
NCBI Official Synonym Symbols
RIC4; SEC9; SNAP; CMS18; RIC-4; SNAP-25; bA416N4.2; dJ1068F16.2
UniProt Protein Name
Synaptosomal-associated protein 25
UniProt Synonym Protein Names
Super protein; SUP; Synaptosomal-associated 25 kDa protein
Protein Family
Synaptosomal-associated protein
UniProt Gene Name
SNAP25
UniProt Synonym Gene Names
SNAP; SNAP-25; SUP
UniProt Entry Name
SNP25_HUMAN
NCBI Protein Information
synaptosomal-associated protein 25
NCBI Summary for SNAP25
Synaptic vesicle membrane docking and fusion is mediated by SNAREs (soluble N-ethylmaleimide-sensitive factor attachment protein receptors) located on the vesicle membrane (v-SNAREs) and the target membrane (t-SNAREs). The assembled v-SNARE/t-SNARE complex consists of a bundle of four helices, one of which is supplied by v-SNARE and the other three by t-SNARE. For t-SNAREs on the plasma membrane, the protein syntaxin supplies one helix and the protein encoded by this gene contributes the other two. Therefore, this gene product is a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. Two alternative transcript variants encoding different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]
Show more UniProt Comments for SNAP25
SNAP-25: a presynaptic plasma membrane protein involved in the regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Part of the SNARE core complex containing SNAP25, VAMP2 and syntaxin 1A. Associates with proteins involved in vesicle docking and membrane fusion. Two alternatively spliced isoforms have been described.
Protein type: Vesicle
Chromosomal Location of Human Ortholog: 20p12-p11.2
Cellular Component: presynaptic membrane; SNARE complex; neuron projection; growth cone; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; trans-Golgi network; cell junction; cytosol; vesicle
Molecular Function: SNAP receptor activity; protein binding; syntaxin-1 binding; calcium-dependent protein binding
Biological Process: synaptic vesicle fusion to presynaptic membrane; synaptic transmission; synaptic vesicle exocytosis; glutamate secretion; neurotransmitter secretion; energy reserve metabolic process; neurotransmitter uptake; pathogenesis; regulation of insulin secretion; synaptic vesicle priming; synaptic vesicle docking during exocytosis
Disease: Myasthenic Syndrome, Congenital, 18
Show more Protein type: Vesicle
Chromosomal Location of Human Ortholog: 20p12-p11.2
Cellular Component: presynaptic membrane; SNARE complex; neuron projection; growth cone; membrane; perinuclear region of cytoplasm; cytoplasm; plasma membrane; trans-Golgi network; cell junction; cytosol; vesicle
Molecular Function: SNAP receptor activity; protein binding; syntaxin-1 binding; calcium-dependent protein binding
Biological Process: synaptic vesicle fusion to presynaptic membrane; synaptic transmission; synaptic vesicle exocytosis; glutamate secretion; neurotransmitter secretion; energy reserve metabolic process; neurotransmitter uptake; pathogenesis; regulation of insulin secretion; synaptic vesicle priming; synaptic vesicle docking during exocytosis
Disease: Myasthenic Syndrome, Congenital, 18
Research Articles on SNAP25
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Pathways associated with SNAP25 elisa kit
| Products by Pathway | Pathway Diagram |
|---|---|
| Acetylcholine Neurotransmitter Release Cycle Pathway antibodies | Acetylcholine Neurotransmitter Release Cycle Pathway Diagram |
| Disease Pathway antibodies | Disease Pathway Diagram |
| Dopamine Neurotransmitter Release Cycle Pathway antibodies | Dopamine Neurotransmitter Release Cycle Pathway Diagram |
| Effects Of Botulinum Toxin Pathway antibodies | Effects Of Botulinum Toxin Pathway Diagram |
| GABA Synthesis, Release, Reuptake And Degradation Pathway antibodies | GABA Synthesis, Release, Reuptake And Degradation Pathway Diagram |
| Glutamate Neurotransmitter Release Cycle Pathway antibodies | Glutamate Neurotransmitter Release Cycle Pathway Diagram |
| Insulin Signaling Pathway antibodies | Insulin Signaling Pathway Diagram |
| Insulin Secretion Pathway antibodies | Insulin Secretion Pathway Diagram |
| Integration Of Energy Metabolism Pathway antibodies | Integration Of Energy Metabolism Pathway Diagram |
| Metabolism Pathway antibodies | Metabolism Pathway Diagram |
Diseases associated with SNAP25 elisa kit
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >127 publications with SNAP25 and Nervous System Diseases |
| Paralysis Antibodies | >22 publications with SNAP25 and Paralysis |
| Cognition Disorders Antibodies | >21 publications with SNAP25 and Cognition Disorders |
| Memory Disorders Antibodies | >20 publications with SNAP25 and Memory Disorders |
| Seizures Antibodies | >17 publications with SNAP25 and Seizures |
| Pain Antibodies | >17 publications with SNAP25 and Pain |
| Learning Disorders Antibodies | >15 publications with SNAP25 and Learning Disorders |
| Neurobehavioral Manifestations Antibodies | >14 publications with SNAP25 and Neurobehavioral Manifestations |
| Movement Disorders Antibodies | >14 publications with SNAP25 and Movement Disorders |
| Diabetes Mellitus Antibodies | >11 publications with SNAP25 and Diabetes Mellitus |
Organs/Tissues associated with SNAP25 elisa kit
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Nerve Antibodies | >668 publications with SNAP25 and Nerve |
| Brain Antibodies | >448 publications with SNAP25 and Brain |
| Pancreas Antibodies | >53 publications with SNAP25 and Pancreas |
| Muscle Antibodies | >46 publications with SNAP25 and Muscle |
| Embryonic Tissue Antibodies | >45 publications with SNAP25 and Embryonic Tissue |
| Eye Antibodies | >37 publications with SNAP25 and Eye |
| Ganglia Antibodies | >30 publications with SNAP25 and Ganglia |
| Pituitary Gland Antibodies | >22 publications with SNAP25 and Pituitary Gland |
| Kidney Antibodies | >17 publications with SNAP25 and Kidney |
| Adrenal Gland Antibodies | >15 publications with SNAP25 and Adrenal Gland |