Principle of the Assay: A capture Antibody highly specific for mIL-6 has been coated to the wells of the microtiter strip plate provided during manufacture. Binding of mIL-6 samples and known standards to the capture antibodies and subsequent binding of the Biotinylated anti-mIL-6 secondary antibody to the analyte is completed during the same incubation period. Any excess unbound analyte and secondary antibody is removed. The HRP conjugate solution is then added to every well including the zero wells, following incubation excess conjugate is removed by careful washing. A chromogen substrate is added to the wells resulting in the progressive development of a blue coloured complex with the conjugate. The colour development is then stopped by the addition of acid turning the resultant final product yellow. The intensity of the produced coloured complex is directly proportional to the concentration of mIL-6 present in the samples and standards. The absorbance of the colour complex is then measured and the generated OD values for each standard are plotted against expected concentration forming a standard curve. This standard curve can then be used to accurately determine the concentration of mIL-6 in any sample tested.

Intended Uses: The Murine IL-6 ELISA kit is a solid phase sandwich ELISA for the in-vitro qualitative and quantitative determination of murine IL-6 (mIL-6) in supernatants, buffered solutions or serum and plasma samples. This assay will recognise both natural and recombinant mIL-6.

Garcia-Perez, M. A. et al., Hum Reprod.,2006; 21(4): 880-7
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Izawa, A. et al. Infect. Immun., 2014 ; 82(5): 1904-1913

Small volumes of IL-6 elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS335514 is a ready-to-use microwell, strip-or-full plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the IL-6, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing IL-6. The ELISA analytical biochemical technique of the MBS335514 kit is based on IL-6 antibody-IL-6 antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect IL-6 antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, IL-6. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).