Mycoplasma Removal Medium (M199)

Mycoplasma is the smallest and simplest prokaryote found so far, with a diameter of 0.1-0.3 um. It can easily pass through the filter membrane and enter the cell culture system. It has various forms such as sphere, rod, filamentous and branching. Usually attached to the surface of the cell membrane, there is no cell wall, so ordinary antibiotics have no effect on it. Mycoplasma contamination is widespread and often underestimated in cell cultures. Mycoplasma contamination can cause many problems, such as changes in cell growth rate, cell morphology, chromosome aberrations, cell membrane antigenicity changes, cell metabolism changes, reduced survival rate after cell resuscitation, and so on, which ultimately leads to inaccurate or incorrect experimental results. Therefore, it is of great significance to clean up mycoplasma contamination in cell culture.

Small volumes of M199 culture media vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS2570332 is a culture media targeting M199. Also known as Mycoplasma Removal Medium (M199).