Principle of the Assay: Nicotinamide adenine dinucleotide phosphate (NADP) is an enzymatic cofactor involved in many redox reactions where it cycles between the reduced (NADPH) and oxidized (NADP) forms. NADP+ is also involved in biosynthetic reactions such as lipid and nucleic acid synthesis where it functions as a reducing agent. The oxidative branch of the pentose phosphate pathway (PPP) is the major source of NADPH produced in animal cells. There is continual interest in monitoring their concentration levels. Quantitative determination of NADP+/NADPH has applications in research pertaining to energy transformation and redox state of cells or tissues. CheKine™ Micro Coenzyme II NADP(H) Assay Kit provides a convenient tool for sensitive detection of the NADP and NADPH, and their ratio in various tissues and subcellular organelles. This Kit is based on enzyme cycling reaction (It does not recognize NADP+/NADPH), in which the formed NADPH reduces a formazan (WST-8) reagent. The intensity of the reduced product color, measured at OD450 nm, is proportionate to the NADP+/NADPH concentration in the sample.

Small volumes of NADP/NADPH assay kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS9711518 is an assay kit targeting NADP/NADPH.