NeoReplace Defined FBS Replacement ,sterile-filtered
Référence NB-58-0177
Conditionnement : 500ml
Marque : Neo Biotech
Contactez votre distributeur local :
Téléphone : +1 850 650 7790
Description
NeoReplace is a defined fetal bovine serum (FBS) replacement for multiple cell types, designed for FBS-like performance with improved reproducibility and control.
It supports growth, adherence, proliferation, viability, and morphology in A549, HeLa, MDCK, Vero, primary immune cells, keratinocytes, and fibroblasts.
Product Features
- Direct FBS substitution at established serum concentrations, usually without stepwise adaptation.
- Defined, homogeneous formulation for consistent culture conditions.
- Compatible with established workflows and multiple cell types.
- Optional Lipid Mix 180 supplementation for increased lipid requirements.
Product Specifications
| Property | Details |
|---|---|
| Appearance | Clear yellow liquid |
| Sizes | 100 mL and 500 mL |
| Sterility | Sterile-filtered |
| Working Concentration | Typically 10%; optimize for the cell type. |
| Culture Types | Adherent and suspension |
| Culture Conditions | 37°C; 5% CO? or established protocol |
| Composition | Proprietary; trace elements, defined proteins, and purified animal-derived protein fractions. |
| Animal Components | Traceable origins; gamma-irradiated for risk mitigation. |
| Intended Use | Research and further manufacturing only |
Storage and Preparation
| Condition | Instructions |
|---|---|
| Storage | ≤ −15°C; protect from light; avoid repeated freeze–thaw cycles. |
| Shipping | Frozen on dry ice |
| Thawing | Overnight at 2–8°C or in a 37°C water bath with gentle shaking. |
| Aliquoting | Gently homogenize; do not vortex. Aseptically prepare 50 mL aliquots. |
| Medium Preparation | 50 mL NeoReplace + 450 mL basal medium; use within 6–8 weeks, protected from light. |
Use and Important Notes
- Do not heat-inactivate.
- Performance depends on basal medium, seeding density, and culture parameters.
- Retain a backup culture during adaptation; use sequential adaptation if necessary.
- Compatible with trypsin detachment; additional trypsin inhibitors are not required. Sensitive cells require an appropriate detachment protocol.
- Not suitable for cryopreservation; use dedicated cryopreservation media.