Neptune Block ELISA Blocking Buffer
Référence MBS258384-5x1L
Conditionnement : 5x1L
Marque : MyBiosource
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Neptune Block ELISA Blocking Buffer Accessory Reagent – Supplied at 1X.
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS258384Description: Neptune Block Blocking Buffer is non-mammalian and designed primarily for antigen-down ELISA formats as well as sandwich assays with high background problems. This buffer is particularly useful when working with human and other mammalian serum… More details
Product Overview
Online Datasheet in PDFProduct Name
Neptune Block ELISA Blocking Buffer, Accessory Reagent
Full Product Name
Neptune Block ELISA Blocking Buffer
Product Synonym Names
Neptune Block ELISA Blocking Buffer
Form/Format
Supplied at 1X.
Preparation and Storage
Store at 2-8 degree C.
pH
7.4 at 1X
Contains
Non-Mammalian Proteins.
Product Categories/Family for Neptune Block ELISA Blocking Buffer accessory reagent
ELISA Solutions; Blocking Buffers
Application Notes for Neptune Block ELISA Blocking Buffer accessory reagent
1. Coat antibody or antigen onto the ELISA plate using ICT's Antibody Coating Buffer or Antigen Coating Buffer.
2. Incubate 8-24 hours at room temperature (RT).
3. Aspirate the coating solution.
4. Wash each well twice with ICT's ELISA Wash Buffer.
5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT.
7. Aspirate the blocking buffer; do not wash.
8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8 degree C.
2. Incubate 8-24 hours at room temperature (RT).
3. Aspirate the coating solution.
4. Wash each well twice with ICT's ELISA Wash Buffer.
5. Block the uncoated regions of the ELISA plate by pipetting 300-400 uL of blocking buffer into each well. Always use an equal or greater volume of blocking buffer than was used for the coating buffer solution.
6. Incubate 8-24 hours at RT. For best blocking, incubate overnight at RT.
7. Aspirate the blocking buffer; do not wash.
8. Run the assay immediately, or dry the plate for long-term storage and seal in a foil storage bag with a desiccant pack. Store dried and packaged plates at 2-8 degree C.
Other Notes
Small volumes of Neptune Block ELISA Blocking Buffer accessory reagent vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Neptune Block ELISA Blocking Buffer purchase
MBS258384 is an accessory reagent targeting Neptune Block ELISA Blocking Buffer.