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> Notch 3 Protein, Human, Recombinant (His)

Notch 3 Protein, Human, Recombinant (His)

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Référence NB-64-55080-20ug

Conditionnement : 20ug

Marque : Neo Biotech

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Notch 3 Protein, Human, Recombinant (His)

(Synonyms: Notch-3, NOTCH3, Notch homolog 3, CADASIL) Copy Product Info

Synonyms: Notch-3, NOTCH3, Notch homolog 3, CADASIL

Catalog No. TMPK-01396 Copy Product Info
Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes. The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Other Forms of Notch 3 Protein, Human, Recombinant (His):
Notch 3 Protein, Human, Recombinant (His & Avi)
Notch 3 Protein, Human, Recombinant (His & Avi), Biotinylated
Notch 3 Protein, Human, Recombinant (hFc)
For research use only—not for human use. No sales to individuals. Use as intended only.

Product Introduction

Bioactivity
Bioactivity
Immobilized Human Notch 3, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Notch 3 Antibody, hFc Tag with the EC50 of 14.2ng/ml determined by ELISA.
Description
Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes. The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Species
Human
Expression System
HEK293 Cells
TagC-His
Accession NumberQ9UM47
ConstructionAla1378-Ser1640
Protein Purity
> 95% as determined by Tris-Bis PAGE; > 95% as determined by HPLC
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS (pH 7.4). Typically, 8% trehalose is incorporated as a protective agent before lyophilization.
ReconstitutionReconstitute the lyophilized protein in distilled water. The product concentration should not be less than 100 μg/ml. Before opening, centrifuge the tube to collect powder at the bottom. After adding the reconstitution buffer, avoid vortexing or pipetting for mixing.
SynonymsNotch-3, NOTCH3, Notch homolog 3, CADASIL
Research Background
Human Notch-3 is part of the Notch family of type I transmembrane glycoproteins involved in a number of early-event developmental processes. The extracellular domain of Notch receptors interact with the extracellular domain of transmembrane ligands Jagged, Delta, and Serrate expressed on the surface of a neighboring cell. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBPJ/RBPSUH and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.
Chemical Properties
Molecular Weight30.18 kDa (predicted). Due to furin-like protease cleavage and glycosylation, the protein migrates to 27-30 kDa and 10-12 kDa based on Tris-Bis PAGE result.
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

NOTCH-3NOTCH 3
Related Tags: Notch 3 Protein, Human, Recombinant (His) chemical structure | Notch 3 Protein, Human, Recombinant (His) molecular weight