Ntrk2 ELISA Kit (Rat)
Référence OKCD01647
Conditionnement : 96Wells
Marque : Aviva Systems Biology
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| Datasheets/Manuals | Printable datasheet for Ntrk2 ELISA Kit (Rat) (OKCD01647) |
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| Predicted Species Reactivity | Rat|Rattus norvegicus | ||||||||||||||||||||||
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| Application | Enzyme-linked Immunosorbent assay-Sandwich | ||||||||||||||||||||||
| ELISA Kit Detection Method | Colorimetric | ||||||||||||||||||||||
| ELISA Kit Duration | 3h | ||||||||||||||||||||||
| ELISA Kit Principle | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tropomyosin Receptor Kinase B (TrkB). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tropomyosin Receptor Kinase B (TrkB). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tropomyosin Receptor Kinase B (TrkB), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Tropomyosin Receptor Kinase B (TrkB) in the samples is then determined by comparing the O.D. of the samples to the standard curve. | ||||||||||||||||||||||
| ELISA Kit Range | 0.156-10ng/mL | ||||||||||||||||||||||
| ELISA Kit Reproducibility | Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Tropomyosin Receptor Kinase B (TrkB) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Tropomyosin Receptor Kinase B (TrkB) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12% | ||||||||||||||||||||||
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| Reconstitution and Storage | 2°C to 8°C|-20°C | ||||||||||||||||||||||
| Sample Type | Tissue homogenates, cell lysates and other biological fluids | ||||||||||||||||||||||
| Sensitivity | < 0.064 ng/mL | ||||||||||||||||||||||
| Specificity | This assay has high sensitivity and excellent specificity for detection of Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2). No significant cross-reactivity or interference between Neurotrophic Tyrosine Kinase Receptor Type 2 (NTRK2) and analogues was observed. | ||||||||||||||||||||||
| Assay Info | Assay Methodology: Quantitative Sandwich Immunoassay |
| Gene Symbol | Ntrk2 |
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| Gene Full Name | neurotrophic receptor tyrosine kinase 2 |
| Alias Symbols | BDNF/NT-3 growth factors receptor;GP145-TrkB/GP95-TrkB;neural receptor protein-tyrosine kinase (trkB);Neurotrophic tyrosine kinase receptor type 2;neurotrophic tyrosine kinase, receptor, type 2;neurotrophic tyrosine receptor kinase type 2;RATTRKB1;Tkrb;trkB;trk-B;trkB tyrosine kinase;TRKB1. |
| NCBI Gene Id | 25054 |
| Protein Name | BDNF/NT-3 growth factors receptor |
| Description of Target | Receptor tyrosine kinase involved in the development and the maturation of the central and the peripheral nervous systems through regulation of neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity. Receptor for BDNF/brain-derived neurotrophic factor and NTF4/neurotrophin-4. Alternatively can also bind NTF3/neurotrophin-3 which is less efficient in activating the receptor but regulates neuron survival through NTRK2. Upon ligand-binding, undergoes homodimerization, autophosphorylation and activation. Recruits, phosphorylates and/or activates several downstream effectors including SHC1, FRS2, SH2B1, SH2B2 and PLCG1 that regulate distinct overlapping signaling cascades. Through SHC1, FRS2, SH2B1, SH2B2 activates the GRB2-Ras-MAPK cascade that regulates for instance neuronal differentiation including neurite outgrowth. Through the same effectors controls the Ras-PI3 kinase-AKT1 signaling cascade that mainly regulates growth and survival. Through PLCG1 and the downstream protein kinase C-regulated pathways controls synaptic plasticity. Thereby, plays a role in learning and memory by regulating both short term synaptic function and long-term potentiation. PLCG1 also leads to NF-Kappa-B activation and the transcription of genes involved in cell survival. Hence, it is able to suppress anoikis, the apoptosis resulting from loss of cell-matrix interactions. May also play a role in neutrophin-dependent calcium signaling in glial cells. |
| Uniprot ID | Q63604 |
| Protein Accession # | NP_001156640.1 |
| Nucleotide Accession # | NM_001163168.2 |
| Protein Size (# AA) | 821 |