Oligomycin A [579-13-5]
Référence T6323-5mg
Conditionnement : 5mg
Marque : TargetMol
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Oligomycin A
(Synonyms: MCH 32) Copy Product Info
Oligomycin A (MCH 32) is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria.
Cas No. 579-13-5
For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:99.99%
Appearance:Solid
Color:White
COA LCMS HNMR
Product Introduction
Bioactivity
Chemical Properties
Storage & Solubility Information
| Description | Oligomycin A (MCH 32) is an inhibitor of ATP synthase, inhibits oxidative phosphorylation and all the ATP-dependent processes occurring on the coupling membrane of mitochondria. |
| Targets & IC50 | K562 cells:0.2 μM, HCT116 cells:0.9 μM, SH-SY5Y cells:29 μM, F0F1-ATPase:1 μM (Ki), HCT116 cells:1 μM |
| In vitro | METHODS: Human melanoma cells WM3734 were treated with Oligomycin A (0.01-1 µg/mL) for 24 h, and lactate production was measured in culture supernatants. RESULTS: Oligomycin A caused an increase in lactate production in WM3734 cells. [1] METHODS: Human lung cancer cells H1299 were treated with Oligomycin A (0.1-1000 ng/mL) for 100 min, and cellular respiration was measured using Oxygen Biosensor System plates. RESULTS: At 100 and 1000 ng/mL, Oligomycin A completely inhibited cellular respiration within approximately one hour. [2] |
| In vivo | METHODS: To assay anti-inflammatory activity, Oligomycin A (0.25 mg/kg) was injected intraperitoneally into C57BL6 mice in a psoriasis model once daily for five days. RESULTS: Oligomycin A reduced ear thickness, keratinocyte proliferation and immune cell infiltration. [3] |
| Synonyms | MCH 32 |
| Cell Research | ATP and Oligomycin Dose-Response Growth Measurement. Cellular ATP changes are measured by CellTiter-Glo reagent. To measure oligomycin dose-response curves, the cells are plated in 96-well plates at about 400–500 cells/well in 100 μl of culture, dosed the next day, and grown for 4 additional days followed by assaying with Cell-Titer-Glo reagent. The dose-response curves are plotted with nonlinear regression analysis of GraphPad Prism.(Only for Reference) |
| Molecular Weight | 791.06 |
| Formula | C45H74O11 |
| Cas No. | 579-13-5 |
| Smiles | 20[C@]12CC[C@@H](CC)\C=C\C=C\C[C@@H](C)[C@H](O)[C@@](C)(O)C(=O)[C@@H](C)[C@H](O)[C@@H](C)C(=O)[C@@H](C)[C@H](O)[C@@H](C)\C=C\C(=O)O[C@](20)([C@@H]1C)[C@H](C)[C@@]1(CC[C@H](C)[C@H](C[C@@H](C)O)O1)O2 |
| Relative Density. | 1.14g/cm3 |
| Storage | Store at low temperature,Keep away from moisture Powder: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. | ||||||||||||||||||||
| Solubility Information | DMSO: 10 mg/mL (12.64 mM), Sonication is recommended. Ethanol: < 1 mg/mL (insoluble or slightly soluble) H2O: < 1 mg/mL (insoluble or slightly soluble) | ||||||||||||||||||||
| In Vivo Formulation | 10% DMSO+40% PEG300+5% Tween 80+45% Saline: 1 mg/mL (1.26 mM), Sonication is recommended. Please add the solvents sequentially, clarifying the solution as much as possible before adding the next one. Dissolve by heating and/or sonication if necessary. Working solution is recommended to be prepared and used immediately. The formulation provided above is for reference purposes only. In vivo formulations may vary and should be modified based on specific experimental conditions. | ||||||||||||||||||||
Solution Preparation Table | |||||||||||||||||||||
DMSO
Note : The dilution table applies only to solid products. For liquid products, please calculate the stock solution based on the stated concentration and/or density. | |||||||||||||||||||||