ONPG – Galactosidase Assay Kit
Référence CA080
Conditionnement : 500assays
Marque : Canvax Biotech
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Téléphone : +1 850 650 7790
ONPG – Galactosidase Assay Kit
Sensitive colorimetric assay kit for β-galactosidase activity using ONPG substrate.
ONPG β-Galactosidase Assay Kit is a colorimetric assay for determining β-galactosidase activity using o-nitrophenyl-β-D-galactopyranoside (ONPG) as substrate. It produces a yellow compound measurable by spectrophotometry.
The assay allows rapid and accurate quantification of enzyme activity in bacterial and cell culture systems, suitable for routine molecular biology experiments.
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Detailed information:
Advantages & Features
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Fast assay: simple workflow with quick readout.
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Sensitive detection: colorimetric measurement at 420 nm.
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Versatile use: compatible with cell lysates and bacterial cultures.
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Quantitative: reliable measurement of enzyme activity.
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Validated performance: reproducible results across assays.
Specifications
Includes
– 10 mL ONGP Substrate solution (4mg/ml)
– 0.5 ml β-mercaptoethanol
– 10 mL Buffer Lysis
– 10 mL Buffer Assay (10x)
– 70 mL Buffer Stop Solution
– 100 μl β-galactosidase enzyme (0.4U/μl)
Download documentation
Datasheet MSDSApplications
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Reporter gene assays.
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Bacterial gene expression analysis.
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Enzyme kinetics studies.
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β-galactosidase activity detection.
Tables & Figures
Quality Control
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Each lot is validated for substrate specificity, sensitivity, and reproducibility using β-galactosidase standards.
Advice
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Perform assays at recommended temperature for optimal enzyme activity.
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Avoid contamination that may cause background color development.
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Use freshly prepared ONPG solution for best performance.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: Store the ONPG substrate solution and β-galactosidase enzyme at –20 °C. All other kit components should be stored at 4 °C.
Citations
- Żyła, N.E. (2025). Dywergencja funkcjonalna i regulatorowa homologów genu BnaHB6 w złożoności sieci transkrypcyjnej w warunkach deficytu wody u rzepaku (Brassica napus L.) [Functional and regulatory divergence of BnaHB6 gene homologs in the complexity of the transcriptional network under water-deficit conditions in rapeseed]. Doctoral thesis, University of Life Sciences.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
Beta-galactosidase assay kit, ONPG β-gal assay, o-nitrophenyl-β-D-galactopyranoside assay, β-gal activity assay, Colorimetric β-galactosidase assay
Also known as:
- Spanish: Kit de ensayo de β-galactosidasa, Ensayo ONPG, Ensayo colorimétrico de β-galactosidasa
- French: Kit d’analyse β-galactosidase, Test ONPG, Essai colorimétrique β-galactosidase
- German: β-Galactosidase-Assaykit, ONPG-Test, Kolorimetrischer β-Galactosidase-Assay
- Italian: Kit saggio β-galattosidasi, Saggio ONPG, Saggio colorimetrico β-galattosidasi
FAQs
Q1: What is the detection principle of this kit?
The assay measures β-galactosidase activity through ONPG hydrolysis at 420 nm.
Q2: Can this kit be used for bacterial cultures?
Yes, it is compatible with bacterial cultures and cell lysates.
Q3: What type of results does the assay provide?
It delivers quantitative and reproducible colorimetric measurements.