RayBio® Human/Mouse/Rat Oxytocin EIA Kit optimized for serum, plasma and cell culture media. Competition-based ELISA on a 96-well strip plate.
Oxytocin ELISA Kit
Oxytocin ELISA Kit
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Product Description
Specifications
| Size | 1 Plate Kit, 2 Plate Kit, 5 Plate Kit |
| Species | Human, Mouse, Rat |
| Sensitivity | 3.6 ng/ml |
| Specificity | This EIA kit is designed to detect human, mouse, and rat Oxytocin |
| Compatible Sample Types | Plasma, Serum |
| Accession Number | P01178 PRO_20495 |
| Gene Id | 5020 |
| Gene Symbols | OXT, OT |
| Protein Name / Synonyms | Oxytocin-neurophysin 1 (OT-NPI) [Cleaved into: Oxytocin (Ocytoci |
| Quantitative/Semi-Quantitative | Quantitative |
| Solid Support | 96-well Microplate |
| Design Principle | Competition-based |
| Method Of Detection | Colorimetric |
| Research Area | Oxytocin Signaling |
| Shipping Type | Blue ice |
| Storage | -20°C |
Introduction
Oxytocin is a nine amino acids peptide hormone and neuropeptide. The active oxytocin is cleaved from oxytocin- neurophysin I precursor. It regulates social bonding and sexual reproduction, playing a role in behaviors from maternal-infant bonding and milk release to empathy, generosity, and orgasm. Oxytocin is released during labor and with stimulation of the nipples from breastfeeding, and causes contraction of the smooth muscle of the uterus and of the mammary gland. This helps with birth, bonding with the baby, and milk production. Oxytocin is also used as a medication to facilitate childbirth.
Product Features
- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
Typical Data
Standard Curve
Application Notes
Kit Components
- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Standard Peptide
- Assay Diluent(s)
- Biotinylated Peptide
- HRP-Streptavidin
- TMB One-Step Substrate
- Stop Solution
- Assay Diagram
- Positive Control Sample
- Capture Antibody
- User Manual
Other Materials Required
- Distilled or deionized water
- Precision pipettes to deliver 2 µl to 1 ml volumes
- Adjustable 1-25 ml pipettes for reagent preparation
- 100 ml and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Orbital shaker
- Aluminum foil
- Saran Wrap
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- SigmaPlot software (or other software that can perform four-parameter logistic regression models)
Protocol Outline
- Prepare all reagents, samples and standards as instructed.
- Add 100 µl detection antibody to each well.
- Incubate 1.5 h at RT or O/N at 4°C.
- Add 100 µl standard or sample to each well.
- Incubate 2.5 h at RT.
- Add 100 µl prepared streptavidin solution.
- Incubate 45 min at RT.
- Add 100 µl TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 µl Stop Solution to each well.
- Read plate at 450 nm immediately.
Storage/Stability
Standard, biotinylated peptide, and positive control should be stored at -20°C after arrival. Avoid multiple freeze-thaws. The remaining kit components may be stored at 4°C. Opened microplate wells and antibody (Item N) may be stored for up to 1 month at 2° to 8°C. Return unused wells to the pouch containing desiccant pack and reseal along entire edge.
citations
Social isolation from early life induces anxiety-like behaviors in adult rats: Relation to neuroendocrine and neurochemical dysfunctions
- Species:
- Rat
- Sample Type:
- Plasma
- Year:
- 2022
- Journal Name:
- Biomedicine & Pharmacotherapy
Loss of MAGEL2 in Prader-Willi syndrome leads to decreased secretory granule and neuropeptide production
- Species:
- Mouse
- Sample Type:
- Serum (cardiac puncture)
- Year:
- 2020
- Journal Name:
- JCI insight
Assessment of the Impact of SARS-CoV-2 Infection on the Sexual Function of Women, Levels of Oxytocin and Prolactin: A Prospective Cohort Study
- Species:
- Human
- Sample Type:
- Plasma (women with SARS-CoV-2 infection)
- Year:
- 2024
- Journal Name:
- Journal of Clinical Medicine
- PubMed ID:
- 38673504
Sex-specific stress and biobehavioral responses to human experimenters in rats
- Species:
- Rat
- Sample Type:
- Plasma
- Year:
- 2022
- Journal Name:
- Frontiers in Neuroscience
The Role of Third Trimester Oxytocin Level in Predicting Postpartum Depression Symptoms among Jordanian Mothers
- Species:
- Human
- Year:
- 2021
- Journal Name:
- Research Square
The effects of 17α-estradiol treatment on endocrine system revealed by single-nucleus transcriptomic sequencing of hypothalamus
- Species:
- Rat
- Sample Type:
- Serum (17α-estradiol-treated rats)
- Year:
- 2025
- Journal Name:
- eLife
- PubMed ID:
- 40996809