PKC beta2, CT (PRKCB, PKCB, PRKCB1, Protein kinase C beta type) (APC)
Référence MBS6334323-5x0,2mL
Conditionnement : 5x0.2mL
Marque : MyBiosource
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The anti-PKC beta2 Pab is used in Western blot to detect PKC beta2 in Jurkat cell lysate (lane 1) and mouse brain tissue lysate (lane 2).
Formalin-fixed and paraffin-embedded human brain tissue reacted with PKC beta2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
PKC Beta2 (PKCB) Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS6334323Protein kinase C (PKC) is a family of serine-and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in… More details
Product Overview
Online Datasheet in PDFProduct Name
PKC beta2 (PKCB), Polyclonal Antibody
Full Product Name
PKC beta2, CT (PRKCB, PKCB, PRKCB1, Protein kinase C beta type) (APC)
Product Gene Name
anti-PKCB antibody
Product Synonym Gene Name
PRKCB
Product Synonym Names
PRKCB; PKCB; PRKCB1; Protein kinase C beta type
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Human, Monkey, Mouse, Rat
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).
Purity/Purification
Purified by Protein G Affinity Chromatography.
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Immunogen
PKC beta2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 644~673 amino acids from the C-terminal region of human PKC beta2.
Conjugate
APC
Note
Preservative Free
Special Handling
Light sensitive
Product Categories/Family for anti-PKCB antibody
Antibodies; Protein Kinases
Applications Tested/Suitable for anti-PKCB antibody
Immunohistochemistry (IHC), FLISA, Western Blot (WB)
Application Notes for anti-PKCB antibody
IHC: 1:10-50
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
FLISA: 1:1,000
WB: 1:100-500
Applications are based on unconjugated antibody.
Immunohistochemistry (IHC) of anti-PKCB antibody
Formalin-fixed and paraffin-embedded human brain tissue reacted with PKC beta2 antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Product References and Citations for anti-PKCB antibody
Chen, D., et al., J. Biol. Chem. 279(6):4829-4839 (2004). Hug, B.A., et al., J. Biol. Chem. 279(2):825-830 (2004). Wu, H., et al., Biochem. Biophys. Res. Commun. 311(4):948-953 (2003). Becker, K.P., et al., J. Biol. Chem. 278(52):52747-52754 (2003). Birikh, K.R., et al., Proc. Natl. Acad. Sci. U.S.A. 100(1):283-288 (2003).
Other Notes
Small volumes of anti-PKCB antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for PKCB purchase
MBS6334323 is an antibody targeting PKCB. Also known as PKC beta2, CT (PRKCB, PKCB, PRKCB1, Protein kinase C beta type) (APC). Reactive in Human, Monkey, Mouse, Rat. Host: Rabbit.
Additional Details
NCBI/Uniprot data below describe general gene information for PKCB. It may not necessarily be applicable to this product.
NCBI Official Synonym Full Names
protein kinase C beta
NCBI Official Symbol
PRKCB
NCBI Official Synonym Symbols
PKCB; PRKCB1; PRKCB2; PKCI(2); PKCbeta; PKC-beta
NCBI Protein Information
protein kinase C beta type
NCBI Summary for PKCB
Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]
Show more Research Articles on PKCB
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.