PKM siRNA (Rat)
Référence MBS828955-30nmol
Conditionnement : 30nmol
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PKM siRNA – Pyruvate Kinase PKM (Rat)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS828955MBS828955 is an siRNA targeting PKM. Also known as PKM siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit PKM expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
PKM, siRNA
Full Product Name
PKM siRNA (Rat)
Product Gene Name
PKM sirna
Product Synonym Names
PKM2; PYKM; Pyruvate kinase PKM; Pyruvate kinase muscle isozyme
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
PKM siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
57,781 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PKM gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for PKM sirna
RNA Interference (RNAi)
Other Notes
Small volumes of PKM sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for PKM. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
pyruvate kinase PKM
NCBI Official Synonym Full Names
pyruvate kinase, muscle
NCBI Official Symbol
Pkm
NCBI Official Synonym Symbols
Pk3; Pkm2; PKM12
UniProt Protein Name
Pyruvate kinase PKM
UniProt Synonym Protein Names
Pyruvate kinase muscle isozyme
Protein Family
Pyruvate kinase
UniProt Gene Name
Pkm
UniProt Synonym Gene Names
Pkm2; Pykm
UniProt Entry Name
KPYM_RAT
NCBI Protein Information
pyruvate kinase PKM
NCBI Summary for PKM
M1 and M2 isozymes of pyruvate kinase; catalyzes the conversion of ATP and pyruvate to ADP and phosphoenolpyruvate in glycolysis [RGD, Feb 2006]
UniProt Comments for PKM
PKM2: one of three rate-limiting enzymes in glycolysis. It catalyzes the transfer of a phosphate group from phosphoenolpyruvate (PEP) to ADP, yielding one molecule ATP and a pyruvate molecule, which is a central metabolic intermediate that can be used as a building block or oxidized further. Overexpressed in a variety of cancers. Interacts with thyroid hormone, and thus may mediate cellular metabolic effects induced by thyroid hormones. Binds Opa protein, a bacterial outer membrane protein involved in gonococcal adherence to and invasion of human cells, suggesting a role of this protein in bacterial pathogens is. There are several mammalian isozymes of pyruvate kinase encoded by different genes. The L type predominates in liver, and the M type in muscle and brain. Two alternatively spliced human isoforms of PKM2 have been reported.
Protein type: Mitochondrial; EC 2.7.1.40; Kinase, other; Nucleotide Metabolism - purine; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glycolysis and gluconeogenesis
Cellular Component: extracellular matrix; mitochondrion; cytoplasm; plasma membrane; nucleus; myelin sheath; vesicle; cilium
Molecular Function: potassium ion binding; protein binding; magnesium ion binding; ADP binding; pyruvate kinase activity; ATP binding
Biological Process: response to gravity; pyruvate biosynthetic process; organ regeneration; glycolysis; programmed cell death; glucose metabolic process; skeletal muscle regeneration; liver development; response to insulin stimulus; response to organic substance; ATP biosynthetic process; response to hypoxia; phosphorylation; response to nutrient; response to muscle inactivity
Show more Protein type: Mitochondrial; EC 2.7.1.40; Kinase, other; Nucleotide Metabolism - purine; Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - glycolysis and gluconeogenesis
Cellular Component: extracellular matrix; mitochondrion; cytoplasm; plasma membrane; nucleus; myelin sheath; vesicle; cilium
Molecular Function: potassium ion binding; protein binding; magnesium ion binding; ADP binding; pyruvate kinase activity; ATP binding
Biological Process: response to gravity; pyruvate biosynthetic process; organ regeneration; glycolysis; programmed cell death; glucose metabolic process; skeletal muscle regeneration; liver development; response to insulin stimulus; response to organic substance; ATP biosynthetic process; response to hypoxia; phosphorylation; response to nutrient; response to muscle inactivity
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with PKM sirna
| Reference Product | PubMed Publications |
|---|---|
| Eno1 sirna | >3 publications with PKM and Eno1 |
| Aldoa sirna | >1 publications with PKM and Aldoa |
| Eno2 sirna | >1 publications with PKM and Eno2 |
Pathways associated with PKM sirna
| Products by Pathway | Pathway Diagram |
|---|---|
| Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies | Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram |
| Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway antibodies | Adenine Ribonucleotide Biosynthesis, IMP => ADP,ATP Pathway Diagram |
| Biosynthesis Of Amino Acids Pathway antibodies | Biosynthesis Of Amino Acids Pathway Diagram |
| Biosynthesis Of Amino Acids Pathway antibodies | Biosynthesis Of Amino Acids Pathway Diagram |
| Biosynthesis Of Antibiotics Pathway antibodies | Biosynthesis Of Antibiotics Pathway Diagram |
| Carbon Metabolism Pathway antibodies | Carbon Metabolism Pathway Diagram |
| Carbon Metabolism Pathway antibodies | Carbon Metabolism Pathway Diagram |
| Central Carbon Metabolism In Cancer Pathway antibodies | Central Carbon Metabolism In Cancer Pathway Diagram |
| Central Carbon Metabolism In Cancer Pathway antibodies | Central Carbon Metabolism In Cancer Pathway Diagram |
| Glucagon Signaling Pathway antibodies | Glucagon Signaling Pathway Diagram |
Diseases associated with PKM sirna
| Disease Name | Pubmed Publications |
|---|---|
| Nervous System Diseases Antibodies | >14 publications with PKM and Nervous System Diseases |
| Fibrosis Antibodies | >8 publications with PKM and Fibrosis |
| Lung Diseases Antibodies | >8 publications with PKM and Lung Diseases |
| Inflammation Antibodies | >7 publications with PKM and Inflammation |
| Cardiovascular Diseases Antibodies | >7 publications with PKM and Cardiovascular Diseases |
| Carcinoma Antibodies | >6 publications with PKM and Carcinoma |
| Cell Transformation, Neoplastic Antibodies | >5 publications with PKM and Cell Transformation, Neoplastic |
| Necrosis Antibodies | >4 publications with PKM and Necrosis |
| Liver Diseases Antibodies | >4 publications with PKM and Liver Diseases |
| Disease Models, Animal Antibodies | >3 publications with PKM and Disease Models, Animal |
Organs/Tissues associated with PKM sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >60 publications with PKM and Brain |
| Muscle Antibodies | >28 publications with PKM and Muscle |
| Kidney Antibodies | >15 publications with PKM and Kidney |
| Liver Antibodies | >15 publications with PKM and Liver |
| Heart Antibodies | >11 publications with PKM and Heart |
| Colon Antibodies | >8 publications with PKM and Colon |
| Lung Antibodies | >7 publications with PKM and Lung |
| Eye Antibodies | >4 publications with PKM and Eye |
| Embryonic Tissue Antibodies | >4 publications with PKM and Embryonic Tissue |
| Dorsal Root Ganglion Antibodies | >3 publications with PKM and Dorsal Root Ganglion |