Conditionnement : 100ug
Téléphone : +1 850 650 7790
Synonyms:
| Description | Pladienolide B is a potent spliceosome inhibitor, a macrolide isolated from Streptomyces obtusususus Mer-12, which targets the SF3B1 subunit of the spliceosome.Pladienolide B exerts its antitumor effects by inhibiting pre-mRNA splicing and inducing necrosis.Pladienolide B possesses antitumor activity and can be used to study leukemia and lymphoid tumors. Pladienolide B has antitumor activity and can be used to study leukemia and lymphoid tumors. |
| In vitro | Pladienolide B (0.1-2 nM; 24-72 h; HeLa cells) decreases SF3b1 expression, induces cell cycle arrest and apoptosis, and suppresses cell viability in human cervical carcinoma cells in a concentration- and time-dependent manner. [3] |
| In vivo | Pladienolide B (2.5-10 mg/kg; i.v.; daily for 5 days) demonstrated strong growth inhibitory or regressive activities against PC-3, OVCAR-3, DU-145, WiDr, and HCT-116, BSY-1 xenografts in BALB/c nu/nu mice, exhibiting intense antitumor activities.[4] |
| Molecular Weight | 536.7 |
| Formula | C30H48O8 |
| Cas No. | 445493-23-2 |
| Smiles | C([C@@H](/C=C/C=C(\C)/[C@@H]1[C@@H](C)/C=C/[C@H](OC(C)=O)[C@](C)(O)CC[C@@H](O)CC(=O)O1)C)[C@@H]2[C@@]([C@@H]([C@H](CC)O)C)(O2)13 |
| Relative Density. | 1.13 g/cm3 (Predicted) |
| Storage | Store at low temperature Pure form: -20°C for 3 years | In solvent: -80°C for 1 year Shipping with blue ice/Shipping at ambient temperature. |
For example, if the intended dosage is 10 mg/kg for animals weighing 20 g , with a dosing volume of 100 μL per animal, and a total of 10 animals are to be administered, using a formulation of 
10% DMSO+ 40% PEG300+ 5% Tween 80+ 45% Saline/PBS/ddH2O , the resulting working solution concentration would be 2 mg/mL.Dissolve 2 mg of the compound in 100 μL DMSO to obtain a stock solution at a concentration of 20 mg/mL . If the required concentration exceeds the compound's known solubility, please contact us for technical support before proceeding.
1) Add 100 μL of the DMSO stock solution to 400 µL PEG300 and mix thoroughly until the solution becomes clear.
2) Add 50 µL Tween 80 and mix well until fully clarified.
3) Add 450 µL Saline,PBS or ddH2O and mix thoroughly until a homogeneous solution is obtained.
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