Principle of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with antigen. Standards or samples are added to the appropriate microtiter plate wells with an antibody specific for ABA. The competitive inhibition reaction is launched between with pre-coated ABA and ABA in samples with the antibody. Then add a Horseradish Peroxidase (HRP) conjugated IgG antibody. A substrate solution is added to the wells and the color develops in opposite to the amount of ABA in the sample. The color development is stopped and the intensity of the color is measured.

Small volumes of ABA elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

MBS703081 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the hormone abscisic acid, ABA, ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ABA. The ELISA analytical biochemical technique of the MBS703081 kit is based on ABA antibody-ABA antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ABA antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ABA. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%). Abscission, the dropping of leaves, is a normal plant function, but premature dropping is indicative of plant pathology. This critical process is regulated by the plant hormone abscisic acid. Although much is known about direct ABA effects. much less is known about it's signaling pathways. Direct quantitation of abscisic acid ABA in plant samples or a sample of overexpressing lysate may be performed with a competitive inhibition ABA ELISA format, similar to CLU ELISA, apolipoprotein APOB ELISA, tri iodothyronine thyroxine T4 ELISA, testosterone ELISA and related products assay formats. Precision protein detection and an uncomplicated protocol make this ELISA kit an ideal purchase. ABA ELiSA kit includes 96 test plate, standard reagent, wash buffers, primary antibody, sheep secondary antibodies and TMB substrate to produce reaction product. Free technical service advice is found in the details included with abscisic acid ABA ELISA kit. Applications include small molecule drug development for pathology.