Polyclonal Antibody to Aspartate Aminotransferase (AST)
Référence MBS2005869-0,5mL
Conditionnement : 0.5mL
Marque : MyBiosource
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Western Blot:
Sample: Recombinant AST, Rat.
Sample: Recombinant AST, Rat.
Western Blot:
Sample:
Lane 1: Rat Brain Tissue;
Lane 2: Rat Heart Tissue;
Lane 3: Rat Liver Tissue;
Lane 4: Rat Kidney Tissue.
Sample:
Lane 1: Rat Brain Tissue;
Lane 2: Rat Heart Tissue;
Lane 3: Rat Liver Tissue;
Lane 4: Rat Kidney Tissue.
DAB staining on IHC-P;
Samples: Rat Testis Tissue.
Samples: Rat Testis Tissue.
DAB staining on IHC-P;
Samples: Rat Kidney Tissue
Samples: Rat Kidney Tissue
DAB staining on IHC-P;
Samples: Rat Intestine Tissue
Samples: Rat Intestine Tissue
FITC staining on IHC-P;
Simple: hela cells)
Simple: hela cells)
DAB staining on IHC-P;
Samples: Rat Liver Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Rat AST Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Samples: Rat Liver Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Rat AST Antibody
Second Ab: 2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047)
Aspartate Aminotransferase (AST) Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2005869SPECIAL PROMOTION: Get FREE Starbucks gift card (Maximum 5 gift cards/product). Gift card(s) will be included with shipment
MBS2005869 is an antibody targeting AST. Also known as Polyclonal Antibody to Aspartate Aminotransferase (AST). Reactive in Rat, Human, Mouse. Host: Rabbit. More details
Product Overview
Online Datasheet in PDFProduct Name
Aspartate Aminotransferase (AST), Polyclonal Antibody
Full Product Name
Polyclonal Antibody to Aspartate Aminotransferase (AST)
Product Gene Name
anti-AST antibody
Clonality
Polyclonal
Host
Rabbit
Species Reactivity
Rat, Human, Mouse
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Concentration
1mg/ml (lot specific)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Matching Pairs
Unconjugated Antibody: AST (MBS2005869)
Biotin Conjugated Antibody: AST (MBS2006326)
Biotin Conjugated Antibody: AST (MBS2006326)
Matching Pairs
Unconjugated Antibody: AST (MBS2005869)
Immunogen: AST (MBS2009670)
Immunogen: AST (MBS2009670)
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Source
Polyclonal antibody preparation
Traits
Liquid
Cross Reactivity
Human; Mouse
Immunogen
Recombinant AST (Met1~Gln413) expressed in E.coli (MBS2009670).
ISO Certification
Manufactured in an ISO 9001:2015 and ISO 13485:2016 Certified Laboratory.
Applications Tested/Suitable for anti-AST antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes for anti-AST antibody
Western blotting: 0.5-2ug/mL;
Immunohistochemistry: 5-20ug/mL;
Immunocytochemistry: 5-20ug/mL;
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL;
Immunocytochemistry: 5-20ug/mL;
Optimal working dilutions must be determined by end user.
Other Notes
Small volumes of anti-AST antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for AST. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
aspartate aminotransferase, cytoplasmic
NCBI Official Synonym Full Names
glutamic-oxaloacetic transaminase 1
NCBI Official Symbol
Got1
NCBI Official Synonym Symbols
ASAT; cCAT; Aspat; Gaspat; cAspAT
UniProt Protein Name
Aspartate aminotransferase, cytoplasmic
UniProt Synonym Protein Names
Cysteine aminotransferase, cytoplasmic; Cysteine transaminase, cytoplasmic; cCAT; Glutamate oxaloacetate transaminase 1; Transaminase A
Protein Family
Allatostatin
UniProt Gene Name
Got1
UniProt Synonym Gene Names
cAspAT; cCAT
NCBI Protein Information
aspartate aminotransferase, cytoplasmic
NCBI Summary for AST
cytosolic aspartate aminotransferase isozyme; enzyme activity in liver and kidney increase in response to glucocorticoids [RGD, Feb 2006]
UniProt Comments for AST
GOT1: Plays a key role in amino acid metabolism. Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family.
Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - tyrosine; EC 2.6.1.1; EC 2.6.1.3; Transferase
Chromosomal Location of Human Ortholog: 1q54
Cellular Component: axon terminus; cytoplasm; cytosol; lysosome; mitochondrion; nucleoplasm; nucleus
Molecular Function: carboxylic acid binding; L-aspartate:2-oxoglutarate aminotransferase activity; L-cysteine:2-oxoglutarate aminotransferase activity; phosphatidylserine decarboxylase activity; pyridoxal phosphate binding
Biological Process: 2-oxoglutarate metabolic process; aspartate biosynthetic process; aspartate catabolic process; aspartate metabolic process; cellular response to insulin stimulus; dicarboxylic acid metabolic process; fatty acid homeostasis; glutamate catabolic process to 2-oxoglutarate; glutamate catabolic process to aspartate; glutamate metabolic process; glycerol biosynthetic process; Notch signaling pathway; oxaloacetate metabolic process; response to glucocorticoid stimulus
Show more Protein type: Amino Acid Metabolism - alanine, aspartate and glutamate; Amino Acid Metabolism - arginine and proline; Amino Acid Metabolism - cysteine and methionine; Amino Acid Metabolism - phenylalanine; Amino Acid Metabolism - phenylalanine, tyrosine and tryptophan biosynthesis; Amino Acid Metabolism - tyrosine; EC 2.6.1.1; EC 2.6.1.3; Transferase
Chromosomal Location of Human Ortholog: 1q54
Cellular Component: axon terminus; cytoplasm; cytosol; lysosome; mitochondrion; nucleoplasm; nucleus
Molecular Function: carboxylic acid binding; L-aspartate:2-oxoglutarate aminotransferase activity; L-cysteine:2-oxoglutarate aminotransferase activity; phosphatidylserine decarboxylase activity; pyridoxal phosphate binding
Biological Process: 2-oxoglutarate metabolic process; aspartate biosynthetic process; aspartate catabolic process; aspartate metabolic process; cellular response to insulin stimulus; dicarboxylic acid metabolic process; fatty acid homeostasis; glutamate catabolic process to 2-oxoglutarate; glutamate catabolic process to aspartate; glutamate metabolic process; glycerol biosynthetic process; Notch signaling pathway; oxaloacetate metabolic process; response to glucocorticoid stimulus
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with anti-AST antibody
| Reference Product | PubMed Publications |
|---|---|
| Mdh1 antibody | >5 publications with AST and Mdh1 |
| Ldhb antibody | >3 publications with AST and Ldhb |
| Mdh2 antibody | >2 publications with AST and Mdh2 |
Pathways associated with anti-AST antibody
| Products by Pathway | Pathway Diagram |
|---|---|
| 2-Oxocarboxylic Acid Metabolism Pathway antibodies | 2-Oxocarboxylic Acid Metabolism Pathway Diagram |
| 2-Oxocarboxylic Acid Metabolism Pathway antibodies | 2-Oxocarboxylic Acid Metabolism Pathway Diagram |
| Alanine And Aspartate Metabolism Pathway antibodies | Alanine And Aspartate Metabolism Pathway Diagram |
| Alanine, Aspartate And Glutamate Metabolism Pathway antibodies | Alanine, Aspartate And Glutamate Metabolism Pathway Diagram |
| Alanine, Aspartate And Glutamate Metabolism Pathway antibodies | Alanine, Aspartate And Glutamate Metabolism Pathway Diagram |
| Amino Acid Synthesis And Interconversion (transamination) Pathway antibodies | Amino Acid Synthesis And Interconversion (transamination) Pathway Diagram |
| Arginine And Proline Metabolism Pathway antibodies | Arginine And Proline Metabolism Pathway Diagram |
| Arginine And Proline Metabolism Pathway antibodies | Arginine And Proline Metabolism Pathway Diagram |
| Arginine Biosynthesis Pathway antibodies | Arginine Biosynthesis Pathway Diagram |
| Arginine Biosynthesis Pathway antibodies | Arginine Biosynthesis Pathway Diagram |
Diseases associated with anti-AST antibody
| Disease Name | Pubmed Publications |
|---|---|
| Disease Models, Animal Antibodies | >6 publications with AST and Disease Models, Animal |
| Nervous System Diseases Antibodies | >6 publications with AST and Nervous System Diseases |
| Lung Neoplasms Antibodies | >6 publications with AST and Lung Neoplasms |
| Liver Diseases Antibodies | >5 publications with AST and Liver Diseases |
| Fatty Liver Antibodies | >3 publications with AST and Fatty Liver |
| Congenital Abnormalities Antibodies | >3 publications with AST and Congenital Abnormalities |
| Cardiovascular Diseases Antibodies | >3 publications with AST and Cardiovascular Diseases |
| Liver Neoplasms Antibodies | >2 publications with AST and Liver Neoplasms |
| Carcinoma, Hepatocellular Antibodies | >2 publications with AST and Carcinoma, Hepatocellular |
| Inflammation Antibodies | >2 publications with AST and Inflammation |
Organs/Tissues associated with anti-AST antibody
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Liver Antibodies | >9 publications with AST and Liver |
| Lung Antibodies | >7 publications with AST and Lung |
| Brain Antibodies | >6 publications with AST and Brain |
| Small Intestine Antibodies | >4 publications with AST and Small Intestine |
| Muscle Antibodies | >4 publications with AST and Muscle |
| Heart Antibodies | >4 publications with AST and Heart |
| Spinal Cord Antibodies | >3 publications with AST and Spinal Cord |
| Artery Antibodies | >2 publications with AST and Artery |
| Olfactory Mucosa Antibodies | >1 publications with AST and Olfactory Mucosa |
| Adrenal Gland Antibodies | >1 publications with AST and Adrenal Gland |
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