Polyclonal Antibody to Microtubule Associated Protein t, MAPT
Figure-1: Immunofluorescent analysis of cortical neuron-glial culture from E20 rat stained with chicken pAb to MAP-Ï„, (34-1067), dilution 1:2,000 in green, and costained with mouse mAb to MAP2,(34-1065), dilution 1:1,000, in red. The blue is DAPI staining of nuclear DNA. The (34-1067) antibody stains perikarya, dendrites and axons of neurons, while MAP2 antibody labels only dendrites and perikarya. As a result, perikarya and dendrites appear orange-yellow, since they contain both MAP2 and tau proteins.
| Format : | Conc. IgY prep. |
| Amount : | 100 µl |
| Isotype : | Chicken, IgY |
| Content : | Antibody is supplied as an aliquot of IgY preparation. |
| Storage condition : | Store the antibody at 4°C; stable for 6 months. For long-term storage; store at -20°C. Avoid repeated freeze and thaw cycles. |
WB: 1:10,000. IF/ICC and IHC: 1:1,000.
For Research Use Only. Not for use in diagnostic/therapeutics procedures.
| Subcellular location: | Cytoplasm, Cell membrane, Cytoplasm, Cell projection, Cytoplasm, Cell projection |
| Post transnational modification: | Glycation of PHF-tau, but not normal brain TAU/MAPT. Glycation is a non-enzymatic post-translational modification that involves a covalent linkage between a sugar and an amino group of a protein molecule forming ketoamine. Subsequent oxidation, fragmentation and/or cross-linking of ketoamine leads to the production of advanced glycation endproducts (AGES). Glycation may play a role in stabilizing PHF aggregation leading to tangle formation in AD. |
| Tissue Specificity: | Expressed in neurons. Isoform PNS-tau is expressed in the peripheral nervous system while the others are expressed in the central nervous system. |
| BioGrid: | 110308. 118 interactions. |
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