PREP siRNA (Rat)
Référence MBS826862-15nmol
Conditionnement : 15nmol
Marque : MyBiosource
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PREP siRNA – Prolyl Endopeptidase (Rat)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS826862MBS826862 is an siRNA targeting PREP. Also known as PREP siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit PREP expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
PREP, siRNA
Full Product Name
PREP siRNA (Rat)
Product Gene Name
PREP sirna
Product Synonym Names
Prolyl endopeptidase; PE; Post-proline cleaving enzyme; rPop
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
PREP siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
80,742 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PREP gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for PREP sirna
RNA Interference (RNAi)
Other Notes
Small volumes of PREP sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for PREP. It may not necessarily be applicable to this product.
NCBI Official Full Name
prolyl endopeptidase
NCBI Official Synonym Full Names
prolyl endopeptidase
NCBI Official Symbol
Prep
NCBI Official Synonym Symbols
rPop
UniProt Protein Name
Prolyl endopeptidase
UniProt Synonym Protein Names
Post-proline cleaving enzyme
Protein Family
Prolyl endopeptidase
UniProt Gene Name
Prep
UniProt Synonym Gene Names
PE
UniProt Entry Name
PPCE_RAT
NCBI Protein Information
prolyl endopeptidase
NCBI Summary for PREP
may play a role in neuropeptide catabolism; expressed in brain; most enzyme activity is localized to the cytosolic fraction [RGD, Feb 2006]
UniProt Comments for PREP
PREP: Cleaves peptide bonds on the C-terminal side of prolyl residues within peptides that are up to approximately 30 amino acids long. Belongs to the peptidase S9A family.
Protein type: Protease; EC 3.4.21.26
Cellular Component: membrane; cytoplasm; nucleus; cytosol
Molecular Function: serine-type endopeptidase activity; endopeptidase activity; peptide binding
Biological Process: proteolysis
Show more Protein type: Protease; EC 3.4.21.26
Cellular Component: membrane; cytoplasm; nucleus; cytosol
Molecular Function: serine-type endopeptidase activity; endopeptidase activity; peptide binding
Biological Process: proteolysis
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Diseases associated with PREP sirna
| Disease Name | Pubmed Publications |
|---|---|
| Carcinoma Antibodies | >113 publications with PREP and Carcinoma |
| Uterine Cervical Neoplasms Antibodies | >55 publications with PREP and Uterine Cervical Neoplasms |
| Cardiovascular Diseases Antibodies | >44 publications with PREP and Cardiovascular Diseases |
| Inflammation Antibodies | >42 publications with PREP and Inflammation |
| Adenocarcinoma Antibodies | >41 publications with PREP and Adenocarcinoma |
| Liver Diseases Antibodies | >28 publications with PREP and Liver Diseases |
| Breast Neoplasms Antibodies | >24 publications with PREP and Breast Neoplasms |
| Neoplasms, Experimental Antibodies | >22 publications with PREP and Neoplasms, Experimental |
| Kidney Diseases Antibodies | >19 publications with PREP and Kidney Diseases |
| Precancerous Conditions Antibodies | >18 publications with PREP and Precancerous Conditions |
Organs/Tissues associated with PREP sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >140 publications with PREP and Brain |
| Lung Antibodies | >53 publications with PREP and Lung |
| Eye Antibodies | >33 publications with PREP and Eye |
| Thymus Antibodies | >19 publications with PREP and Thymus |
| Pancreas Antibodies | >19 publications with PREP and Pancreas |
| Spleen Antibodies | >18 publications with PREP and Spleen |
| Embryonic Tissue Antibodies | >18 publications with PREP and Embryonic Tissue |
| Ovary Antibodies | >9 publications with PREP and Ovary |
| Adrenal Gland Antibodies | >7 publications with PREP and Adrenal Gland |
| Adipose Tissue Antibodies | >6 publications with PREP and Adipose Tissue |