Prion PrPsc, mAb 1.5D7
Référence MBS584126-0,1mg
Conditionnement : 0.1mg
Marque : MyBiosource
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N.D.= Not Determined; IHC = Immuno histochemistry; F = Frozen sections; P = Paraffin sections; IF = Immuno Fluorescence; FC = Flow Cytometry; FS = Functional Studies; IA = Immuno Assays; IP = Immuno Precipitation; W = Western blot
Prion Prpsc Antibody – Mouse Monoclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS584126The monoclonal antibody 1.5D7 recognizes the disease associated isoform of the prion protein termed PrPSc. Prion diseases, also known as spongiform encephalopathies, are a group of neurodegenerative diseases that include BSE (bovine spongiform encephalopathy)… More details
Product Overview
Online Datasheet in PDFProduct Name
Prion PrPsc, Monoclonal Antibody
Full Product Name
Prion PrPsc, mAb 1.5D7
Product Synonym Names
Spongiform encephalopathies
Clonality
Monoclonal
Isotype
IgG2b
Clone Number
1.5D7
Host
Mouse
Form/Format
0.2 um filtered in PBS+0.1%BSA+0.02%NaN3
Purity/Purification
Protein G
Concentration
100 ug/mL (lot specific)
Preparation and Storage
Product should be stored at 4°C. Under recommended storage conditions, product is stable for at least one year.
Conjugate
None
Cross reactivity
Human, Mouse, Bovine, Hamster, Ovine.
Applications Tested/Suitable for anti-Prion PrPsc antibody
Immunohistochemistry-Frozen (IHC-F), Immunohistochemistry-Paraffin (IHC-P), Immunoassay (IA), Western Blot (WB)
Application Notes for anti-Prion PrPsc antibody
Dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50.
- WB: A reduced sample treatment and SDS-Page was used. The band size is 30 kDa (Ref.1).
- IHC-P: Paraffin sections were deparaffinized, rehydrated and endogenous peroxidase was quenched using 0.3% H2O2 in methanol for 20 min. As positive control BSE infected brain tissue was used and as negative control non-diseased brain tissue (Ref.1).
- Positive control: Prion diseased brain tissue; Negative control: non-diseased brain tissue.
- WB: A reduced sample treatment and SDS-Page was used. The band size is 30 kDa (Ref.1).
- IHC-P: Paraffin sections were deparaffinized, rehydrated and endogenous peroxidase was quenched using 0.3% H2O2 in methanol for 20 min. As positive control BSE infected brain tissue was used and as negative control non-diseased brain tissue (Ref.1).
- Positive control: Prion diseased brain tissue; Negative control: non-diseased brain tissue.
Product References and Citations for anti-Prion PrPsc antibody
1. Cordes, H et al. Characterisation of new monoclonal antibodies reacting with prions from both human and animal brain tissues. J Immunol Method 2008, 337: 106.
2. Bergstr¶m, A et al; Short-term study of the uptake of PrPSc by the Peyers patches in hamsters after oral exposure to scrapie. J Comp Pathol 2006, 134: 126
2. Bergstr¶m, A et al; Short-term study of the uptake of PrPSc by the Peyers patches in hamsters after oral exposure to scrapie. J Comp Pathol 2006, 134: 126
Other Notes
Small volumes of anti-Prion PrPsc antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Searchable Terms for Prion PrPsc purchase
MBS584126 is an antibody targeting Prion PrPsc. Also known as Prion PrPsc, mAb 1.5D7. Host: Mouse.
Additional Details
NCBI/Uniprot data below describe general gene information for Prion PrPsc. It may not necessarily be applicable to this product.
NCBI GI #
NCBI Accession #
NCBI Official Full Name
PrPSc=scrapie prion protein
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.