PTN siRNA (Rat)
Référence MBS828181-15nmol
Conditionnement : 15nmol
Marque : MyBiosource
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PTN siRNA – Pleiotrophin (Rat)
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS828181MBS828181 is an siRNA targeting PTN. Also known as PTN siRNA (Rat). Reactive in Rat. Host: Synthetic. siRNA to inhibit PTN expression using RNA interference More details
Product Overview
Online Datasheet in PDFProduct Name
PTN, siRNA
Full Product Name
PTN siRNA (Rat)
Product Gene Name
PTN sirna
Product Synonym Names
Pleiotrophin; PTN; Heparin-binding brain mitogen; HBBM; Heparin-binding growth factor 8; HBGF-8; Heparin-binding growth-associated molecule; HB-GAM; Heparin-binding neutrophic factor; HBNF; Osteoblast-specific factor 1; OSF-1
Host
Synthetic
Species Reactivity
Rat
Form/Format
Lyophilized powder
Purity/Purification
> 97%
Specificity
PTN siRNA (Rat) is a target-specific 19-23 nt siRNA oligo duplexes designed to knock down gene expression.
Preparation and Storage
Shipped at 4 degree C. Store at -20 degree C for one year.
Molecular Weight
18,869 Da
Negative Control
siRNA Negative Control (Catalog# MBS8241404) is a non-targeting 21 nt siRNA recommended as a negative control for experiments using targeted siRNA transfection.
Quality Control
Oligonucleotide synthesis is monitored base by base through trityl analysis to ensure appropriate coupling efficiency. The oligo is subsequently purified by affinity-solid phase extraction. The annealed RNA duplex is further analyzed by mass spectrometry to verify the exact composition of the duplex. Each lot is compared to the previous lot by mass spectrometry to ensure maximum lot-to-lot consistency.
Directions for Use
We recommends transfection with 100 nM siRNA 48 to 72 hours prior to cell lysis. Before resuspending, briefly centrifuge the tube to ensure the lyophilized siRNA is at the bottom of the tube. Resuspend the siRNA oligos to an appropriate concentration with DEPC water. For each vial, suitable for 250 transfections in 24 well plate (20 pmol for each well).
Components
We offer pre-designed sets of 3 different target-specific siRNA oligo duplexes of rat PTN gene. Each vial contains 5 nmol of lyophilized siRNA. The duplexes can be transfected individually or pooled together to achieve knockdown of the target gene, which is most commonly assessed by qPCR or western blot. Our siRNA oligos are also chemically modified (2'-OMe) at no extra charge for increased stability and enhanced knockdown in vitro and in vivo.
Applications Tested/Suitable for PTN sirna
RNA Interference (RNAi)
Other Notes
Small volumes of PTN sirna vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.
Additional Details
NCBI/Uniprot data below describe general gene information for PTN. It may not necessarily be applicable to this product.
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
GenBank Nucleotide #
NCBI Official Full Name
pleiotrophin
NCBI Official Synonym Full Names
pleiotrophin
NCBI Official Symbol
Ptn
NCBI Official Synonym Symbols
HARP; Hbnf
UniProt Protein Name
Pleiotrophin
UniProt Synonym Protein Names
Heparin-binding brain mitogen; HBBM; Heparin-binding growth factor 8; HBGF-8; Heparin-binding growth-associated molecule; HB-GAM; Heparin-binding neutrophic factor; HBNF; Osteoblast-specific factor 1; OSF-1
Protein Family
Pleiotrophin
UniProt Gene Name
Ptn
UniProt Synonym Gene Names
PTN; HBBM; HBGF-8; HB-GAM; HBNF; OSF-1
UniProt Entry Name
PTN_RAT
NCBI Protein Information
pleiotrophin
NCBI Summary for PTN
binds heparin; may act as a growth factor; may play a role in liver regeneration and fibrinogenesis [RGD, Feb 2006]
UniProt Comments for PTN
PTN: Secreted growth factor that induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells. Binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti- apoptotic signaling of PTN and regulation of cell proliferation. Belongs to the pleiotrophin family.
Protein type: Secreted; Secreted, signal peptide
Cellular Component: proteinaceous extracellular matrix; endoplasmic reticulum; extracellular region
Molecular Function: heparin binding; growth factor activity
Biological Process: ossification; positive regulation of cell division; learning; bone mineralization
Show more Protein type: Secreted; Secreted, signal peptide
Cellular Component: proteinaceous extracellular matrix; endoplasmic reticulum; extracellular region
Molecular Function: heparin binding; growth factor activity
Biological Process: ossification; positive regulation of cell division; learning; bone mineralization
Precautions
All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.
Disclaimer
While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.
Product Note
Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.
Product PubMed Publications
Products associated with PTN sirna
| Reference Product | PubMed Publications |
|---|---|
| Alk sirna | >25 publications with PTN and Alk |
| Ptprz1 sirna | >20 publications with PTN and Ptprz1 |
| Sdc3 sirna | >7 publications with PTN and Sdc3 |
| Src sirna | >6 publications with PTN and Src |
| Fyn sirna | >4 publications with PTN and Fyn |
| Pten sirna | >3 publications with PTN and Pten |
| Cspg5 sirna | >1 publications with PTN and Cspg5 |
Diseases associated with PTN sirna
| Disease Name | Pubmed Publications |
|---|---|
| Carcinoma Antibodies | >64 publications with PTN and Carcinoma |
| Brain Diseases Antibodies | >50 publications with PTN and Brain Diseases |
| Cardiovascular Diseases Antibodies | >32 publications with PTN and Cardiovascular Diseases |
| Disease Models, Animal Antibodies | >29 publications with PTN and Disease Models, Animal |
| Lung Neoplasms Antibodies | >22 publications with PTN and Lung Neoplasms |
| Neoplasm Metastasis Antibodies | >20 publications with PTN and Neoplasm Metastasis |
| Inflammation Antibodies | >19 publications with PTN and Inflammation |
| Necrosis Antibodies | >15 publications with PTN and Necrosis |
| Heart Diseases Antibodies | >14 publications with PTN and Heart Diseases |
| Neoplasms, Experimental Antibodies | >14 publications with PTN and Neoplasms, Experimental |
Organs/Tissues associated with PTN sirna
| Organ/Tissue Name | Pubmed Publications |
|---|---|
| Brain Antibodies | >200 publications with PTN and Brain |
| Muscle Antibodies | >61 publications with PTN and Muscle |
| Embryonic Tissue Antibodies | >35 publications with PTN and Embryonic Tissue |
| Lung Antibodies | >26 publications with PTN and Lung |
| Heart Antibodies | >23 publications with PTN and Heart |
| Kidney Antibodies | >20 publications with PTN and Kidney |
| Connective Tissue Antibodies | >19 publications with PTN and Connective Tissue |
| Liver Antibodies | >19 publications with PTN and Liver |
| Prostate Antibodies | >12 publications with PTN and Prostate |
| Dorsal Root Ganglion Antibodies | >11 publications with PTN and Dorsal Root Ganglion |