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> PU.1/SPI1 Protein, Human, Recombinant (His)

PU.1/SPI1 Protein, Human, Recombinant (His)

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Référence NB-64-61702-10ug

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PU.1/SPI1 Protein, Human, Recombinant (His)

(Synonyms: spleen focus forming virus (SFFV) proviral integration oncogene, SPI-A, SPI-1, SFPI1, PU.1, OF) Copy Product Info

Synonyms: spleen focus forming virus (SFFV) proviral integration oncogene, SPI-A, SPI-1, SFPI1, PU.1, OF

Catalog No. TMPY-06917 Copy Product Info
PU.1/SPI1 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 32.04 kDa and the accession number is P17947.
Other Forms of PU.1/SPI1 Protein, Human, Recombinant (His):
PU.1/SPI1 Protein, Mouse, Recombinant (His)TMPY-06912
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For research use only—not for human use. No sales to individuals. Use as intended only.
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Purity:≥ 90 % as determined by SDS-PAGE.
Appearance:Lyophilized powder
Color:White
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Product Introduction

Bioactivity
Bioactivity
Activity testing is in progress. It is theoretically active, but we cannot guarantee it. If you require protein activity, we recommend choosing the eukaryotic expression version first.
Description
PU.1/SPI1 Protein, Human, Recombinant (His) is expressed in E. coli expression system with His tag. The predicted molecular weight is 32.04 kDa and the accession number is P17947.
Species
Human
Expression System
E. coli
TagN-His
Accession NumberP17947
ConstructionA DNA sequence encoding the Human SPI1 (P17947) (Met1-His270) was expressed, with a polyhistidine tag at the N-terminus. Predicted N terminal: Met
Protein Purity
≥ 90 % as determined by SDS-PAGE.
Endotoxin< 1.0 EU/μg of the protein as determined by the LAL method.
FormulationLyophilized from a solution filtered through a 0.22 μm filter, containing PBS, 0.05% SKL. Typically, a mixture containing 5% to 8% trehalose, mannitol, and 0.01% Tween 80 is incorporated as a protective agent before lyophilization.
ReconstitutionA Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information.
Synonymsspleen focus forming virus (SFFV) proviral integration oncogene, SPI-A, SPI-1, SFPI1, PU.1, OF
Research Background
SPI1 (also known as PU.1) is a dominant but transient regulator in early T-cell precursors and a potent transcriptional controller of developmentally important pro-T-cell genes. SPI1 is an essential transcription factor (TF) for the hematopoietic lineage, in which its expression is tightly controlled through a -17-kb upstream regulatory region and a promoter region. The E26 transformation-specific (ETS) family transcription factor PU.1/Spi1 acts as a master regulator of myeloid and lymphoid development. PU.1-deficient mice show a complete loss of microglia, indicating that PU.1 plays a pivotal role in micro-gliogenesis. The Spi1/PU.1 transcription factor is a key regulator of many steps of hematopoiesis, and limits self-renewal of hematopoietic stem cells. The deregulation of its expression or activity contributes to leukemia, in which Spi1 can be either an oncogene or a tumor suppressor.
Chemical Properties
Molecular Weight32.04 kDa (predicted)
Storage & Solubility Information
ShippingIn general, lyophilized powders are shipped with blue ice, while solutions are shipped with dry ice.
StorageIt is recommended to store recombinant proteins at -20°C to -80°C for future use. Lyophilized powders can be stably stored for over 12 months, while liquid products can be stored for 6-12 months at -80°C. For reconstituted protein solutions, the solution can be stored at -20°C to -80°C for at least 3 months. Please avoid multiple freeze-thaw cycles and store products in aliquots.

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Keywords

SPI1SPI 1SFPI-1SFPI 1
Related Tags: PU.1/SPI1 Protein, Human, Recombinant (His) chemical structure | PU.1/SPI1 Protein, Human, Recombinant (His) molecular weight