Rabbit Angiotensin I Converting Enzyme (ACE) ELISA Kit

Référence MBS2700710-96

Conditionnement : 96-Strip-Wells

Marque : MyBiosource

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Angiotensin 1 Converting Enzyme (ACE) ELISA Kit for Rabbit

For Research Use Only. Not for use in diagnostic procedures. Special Promotion Catalog #: MBS2700710
SPECIAL PROMOTIONAL PRICING for a limited time

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Angiotensin I Converting Enzyme (ACE). Standards or samples are then added to the appropriate… More details

Product Overview

Online Datasheet in PDF
Product Name
Angiotensin I Converting Enzyme (ACE), ELISA Kit
Full Product Name
Rabbit Angiotensin I Converting Enzyme (ACE) ELISA Kit
Product Gene Name
ACE elisa kit
Product Synonym Names
CD143; ACE1; DCP1; ACEI; ACE-I; Kininase II; Angiotensin-Converting Enzyme; Peptidyl-Dipeptidase A; Dipeptidyl Carboxypeptidase 1; Angiotensin-converting enzyme, soluble form
Species Reactivity
Rabbit
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma and Other Biological Fluids
Detection Range
0.312-20ng/mL
Sensitivity
< 0.128ng/mL
Specificity
This assay has high sensitivity and excellent specificity for detection of Angiotensin I Converting Enzyme (ACE).
No significant cross-reactivity or interference between Angiotensin I Converting Enzyme (ACE) and analogues was observed.
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Molecular Weight
78,694 Da
OMIM
J04144 mRNA
3D Structure
ModBase 3D Structure for P12821
Supply Chain Verification
Manufactured in a lab with traceable raw materials. Bulk orders can typically be prepared to the customer’s specifications, please inquire.
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Angiotensin I Converting Enzyme (ACE) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
ISO Certification
Manufactured in an ISO 9001:2008 and ISO 13485:2003 Certified Laboratory.

Typical Testing Data/Standard Curve (for reference only) of ACE elisa kit

Rabbit ACE ELISA Kit – Cat# MBS2700710

Other Notes

Small volumes of ACE elisa kit vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.

Searchable Terms for ACE purchase

MBS2700710 is a ready-to-use microwell, strip plate ELISA (enzyme-linked immunosorbent assay) Kit for analyzing the presence of the Angiotensin I Converting Enzyme (ACE) ELISA Kit target analytes in biological samples. The concentration gradients of the kit standards or positive controls render a theoretical kit detection range in biological research samples containing ACE. The ELISA analytical biochemical technique of the MBS2700710 kit is based on ACE antibody-ACE antigen interactions (immunosorbency) and an HRP colorimetric detection system to detect ACE antigen targets in samples. The ELISA Kit is designed to detect native, not recombinant, ACE. Appropriate sample types may include undiluted body fluids and/or tissue homogenates, secretions. Quality control assays assessing reproducibility identified the intra-assay CV (%) and inter-assay CV(%).

Additional Details

NCBI/Uniprot data below describe general gene information for ACE. It may not necessarily be applicable to this product.

NCBI GI #
NCBI GeneID
NCBI Accession #
UniProt Primary Accession #
UniProt Secondary Accession #
UniProt Related Accession #
NCBI Official Full Name
angiotensin I converting enzyme, partial
NCBI Official Synonym Full Names
angiotensin I converting enzyme
NCBI Official Symbol
ACE
NCBI Official Synonym Symbols
DCP; ACE1; DCP1; CD143
UniProt Protein Name
Angiotensin-converting enzyme
UniProt Synonym Protein Names
Dipeptidyl carboxypeptidase I; Kininase II; CD_antigen: CD143
Protein Family
Accessory cholera enterotoxin
UniProt Gene Name
ACE
UniProt Synonym Gene Names
DCP; DCP1; ACE
NCBI Protein Information
angiotensin-converting enzyme

NCBI Summary for ACE

This gene encodes an enzyme involved in catalyzing the conversion of angiotensin I into a physiologically active peptide angiotensin II. Angiotensin II is a potent vasopressor and aldosterone-stimulating peptide that controls blood pressure and fluid-electrolyte balance. This enzyme plays a key role in the renin-angiotensin system. Many studies have associated the presence or absence of a 287 bp Alu repeat element in this gene with the levels of circulating enzyme or cardiovascular pathophysiologies. Multiple alternatively spliced transcript variants encoding different isoforms have been identified, and two most abundant spliced variants encode the somatic form and the testicular form, respectively, that are equally active. [provided by RefSeq, May 2010]
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UniProt Comments for ACE

Converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. Also able to inactivate bradykinin, a potent vasodilator. Has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety.
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Precautions

All of MyBioSource's Products are for scientific laboratory research purposes and are not for diagnostic, therapeutics, prophylactic or in vivo use. Through your purchase, you expressly represent and warrant to MyBioSource that you will properly test and use any Products purchased from MyBioSource in accordance with industry standards. MyBioSource and its authorized distributors reserve the right to refuse to process any order where we reasonably believe that the intended use will fall outside of our acceptable guidelines.

Disclaimer

While every efforts were made to ensure the accuracy of the information provided in this datasheet, MyBioSource will not be liable for any omissions or errors contained herein. MyBioSource reserves the right to make changes to this datasheet at any time without prior notice.

It is the responsibility of the customer to report product performance issues to MyBioSource within 30 days of receipt of the product. Please visit our Terms & Conditions page for more information.

Product Note

Select online data sheet information is drawn from bioinformatics databases, occasionally resulting in ambiguous or non-relevant product information. It is the responsibility of the customer to review, verify, and evaluate the information to make sure it matches their requirements before purchasing the kit. Our ELISA Kit assays are dynamic research tools and sometimes they may be updated and improved. If the format of this assay is important to you then please request the current manual or contact our technical support team with a presales inquiry before placing an order. We will confirm the current details of the assay. We cannot guarantee the sample manual posted online is the most current manual, it is intended to serve as an example only. Please refer to the instructions For Use provided with the assay kit for precise details.

Product PubMed Publications

Products associated with ACE elisa kit

 Reference Product  PubMed Publications
 AGT elisa kit  >419 publications with ACE and AGT
 ACE2 elisa kit  >402 publications with ACE and ACE2
 SERPINE1 elisa kit  >188 publications with ACE and SERPINE1
 NOS3 elisa kit  >142 publications with ACE and NOS3
 REN elisa kit  >129 publications with ACE and REN
 FOS elisa kit  >42 publications with ACE and FOS
 CTGF elisa kit  >19 publications with ACE and CTGF
 VEGFA elisa kit  >14 publications with ACE and VEGFA
 ATP6AP2 elisa kit  >8 publications with ACE and ATP6AP2
 KNG1 elisa kit  >1 publications with ACE and KNG1

Pathways associated with ACE elisa kit

 Products by Pathway  Pathway Diagram
 ACE Inhibitor Pathway antibodies  ACE Inhibitor Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Chagas Disease (American Trypanosomiasis) Pathway antibodies  Chagas Disease (American Trypanosomiasis) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Hypertrophic Cardiomyopathy (HCM) Pathway antibodies  Hypertrophic Cardiomyopathy (HCM) Pathway Diagram
 Metabolism Of Angiotensinogen To Angiotensins Pathway antibodies  Metabolism Of Angiotensinogen To Angiotensins Pathway Diagram
 Metabolism Of Proteins Pathway antibodies  Metabolism Of Proteins Pathway Diagram
 Peptide Hormone Metabolism Pathway antibodies  Peptide Hormone Metabolism Pathway Diagram
 Renin-angiotensin System Pathway antibodies  Renin-angiotensin System Pathway Diagram
 Renin-angiotensin System Pathway antibodies  Renin-angiotensin System Pathway Diagram

Diseases associated with ACE elisa kit

 Disease Name  Pubmed Publications
 Cardiovascular Diseases Antibodies  >13297 publications with ACE and Cardiovascular Diseases
 Hypertension Antibodies  >8533 publications with ACE and Hypertension
 Heart Failure Antibodies  >4505 publications with ACE and Heart Failure
 Kidney Diseases Antibodies  >3354 publications with ACE and Kidney Diseases
 Myocardial Infarction Antibodies  >2499 publications with ACE and Myocardial Infarction
 Coronary Artery Disease Antibodies  >2137 publications with ACE and Coronary Artery Disease
 Coronary Disease Antibodies  >2121 publications with ACE and Coronary Disease
 Stroke Antibodies  >1350 publications with ACE and Stroke
 Renal Insufficiency Antibodies  >1269 publications with ACE and Renal Insufficiency
 Respiratory Tract Diseases Antibodies  >1232 publications with ACE and Respiratory Tract Diseases

Organs/Tissues associated with ACE elisa kit

 Organ/Tissue Name  Pubmed Publications
 Blood Antibodies  >11695 publications with ACE and Blood
 Heart Antibodies  >8012 publications with ACE and Heart
 Vascular Antibodies  >4442 publications with ACE and Vascular
 Kidney Antibodies  >4070 publications with ACE and Kidney
 Muscle Antibodies  >2114 publications with ACE and Muscle
 Lung Antibodies  >1536 publications with ACE and Lung
 Brain Antibodies  >1229 publications with ACE and Brain
 Intestine Antibodies  >264 publications with ACE and Intestine
 Eye Antibodies  >228 publications with ACE and Eye
 Testis Antibodies  >223 publications with ACE and Testis