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> Rabbit Anti-ATP6V1B1 Polyclonal Antibody

Rabbit Anti-ATP6V1B1 Polyclonal Antibody

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Référence HPA031847

Conditionnement : 100ul

Marque : Atlas Antibodies

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HPA031847
ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1

Anti-ATP6V1B1 Antibody

Polyclonal Antibody against HUMAN ATP6V1B1

0.1 mg/ml
Anti-ATP6V1B1 Antibody
Anti-ATP6V1B1 Antibody
Anti-ATP6V1B1 Antibody
Anti-ATP6V1B1 Antibody
Anti-ATP6V1B1 Antibody
Anti-ATP6V1B1 Antibody
Anti-ATP6V1B1 Antibody
Anti-ATP6V1B1 Antibody
Anti-ATP6V1B1 Antibody
Anti-ATP6V1B1 Antibody
Anti-ATP6V1B1 Antibody
Anti-ATP6V1B1 Antibody
Anti-ATP6V1B1 Antibody
info@biovalley.fr

Immunohistochemistry analysis in human kidney and liver tissues using HPA031847 antibody. Corresponding ATP6V1B1 RNA-seq data are presented for the same tissues.

Product details

  • Target protein: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
  • Target gene: ATP6V1B1
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000006269 (79%)RAT - ENSRNOG00000013573 (77%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000062834
  • Concentration: 0.1 mg/ml

Applications

  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:50 - 1:200
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

  • Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.  
  • Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above) 

Target information

  • Protein name: ATPase, H+ transporting, lysosomal 56/58kDa, V1 subunit B1
  • Gene name: ATP6V1B1
  • Alternative gene names: ATP6B1, RTA1B, VATB, Vma2, VPP3, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (4)

  • Han GH, Yun H, Chung JY, Kim JH, Cho HOncol Rep None2023 Mar 31
  • Zachar R, Thiel S, Hansen S, Henriksen ML, Skjoedt MO, Skjodt K, Hamzaei Z, Madsen K, Lund L, Hummler E, Svenningsen P, Jensen BLSci Rep , 2022 Sep 24; 12:15955. Epub 2022 Sep 242022 Sep 24

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