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Réactifs et instruments pour l'immunologie, la biologie cellulaire et la biologie moléculaire.

Rabbit Anti-BARHL1 Polyclonal Antibody

Imprimer

Conditionnement : 100ul

Téléphone : +1 850 650 7790

Demander plus d'informations

Téléphone : +1 850 650 7790

HPA004809

BarH-like homeobox 1

Anti-BARHL1 Antibody
- ChIP-Certified

Polyclonal Antibody against HUMAN BARHL1

0.4 mg/ml

info@biovalley.fr

Immunohistochemical staining of mouse embryo E14 shows strong nuclear positivity in neuronal cells in developing brain stem and cerebellum.

Target protein: BarH-like homeobox 1
Target gene: BARHL1
Verified species reactivity: Human, Mouse
Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000026805 (96%)RAT - ENSRNOG00000013209 (96%)
Clonality: Polyclonal
Isotype: IgG
Host: Rabbit
Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
Material Safety Data Sheet
Purification method: Affinity purified using the PrEST antigen as affinity ligand
Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Chromatin Immunoprecipitation (ChIP) recommended conditions
- Working concentration: 1-10µg per reaction
Immunohistochemistry (IHC) recommended conditions
- Dilution: 1:200 - 1:500
- Retrieval method: HIER pH6

Chromatin Immunoprecipitation (ChIP)Validated for use in ChIP-Exo-Seq and other ChIP-based assays via chromatin immunoprecipitation followed by λ exonuclease digestion and next-generation DNA sequencing to precisely map protein-DNA interactions at single base pair resolution. *Please note not all antibody batches are validated for use in ChIP applications.
Immunohistochemistry (IHC)

Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Amin ND, Kelley KW, Kaganovsky K, Onesto M, Hao J, Miura Y, McQueen JP, Reis N, Narazaki G, Li T, Kulkarni S, Pavlov S, Pașca SPCell Stem Cell , 2024 Dec 5; 31(12):1831-1846.e92024 Dec 5
Rekler D, Ofek S, Kagan S, Friedlander G, Kalcheim CDevelopment , 2024 Sep 30; 151(19):dev202973. Epub 2024 Sep 302024 Sep 30