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> Rabbit Anti-CPSF2 Polyclonal Antibody

Rabbit Anti-CPSF2 Polyclonal Antibody

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Référence HPA024238

Conditionnement : 100ul

Marque : Atlas Antibodies

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HPA024238
cleavage and polyadenylation specific factor 2, 100kDa

Anti-CPSF2 Antibody

Polyclonal Antibody against HUMAN CPSF2

0.4 mg/ml
Anti-CPSF2 Antibody
Anti-CPSF2 Antibody
Anti-CPSF2 Antibody
Anti-CPSF2 Antibody
Anti-CPSF2 Antibody
Anti-CPSF2 Antibody
Anti-CPSF2 Antibody
Anti-CPSF2 Antibody
Anti-CPSF2 Antibody
info@biovalley.fr

Immunohistochemical staining of human cerebral cortex shows strong nuclear positivity in neuronal and glial cells.

Product details

  • Target protein: cleavage and polyadenylation specific factor 2, 100kDa
  • Target gene: CPSF2
  • Verified species reactivity: Human, Mouse, Rat
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000041781 (96%)RAT - ENSRNOG00000005733 (95%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: R11045
  • Concentration: 0.4 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:500 - 1:1000
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Immunohistochemistry (IHC) 
  • Western Blot (WB)Validated in Western blot using relevant lysates (see Western blot application images above) 

Target information

  • Protein name: cleavage and polyadenylation specific factor 2, 100kDa
  • Gene name: CPSF2
  • Alternative gene names: KIAA1367, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (3)

  • Russo M, Piccolo V, Polizzese D, Prosperini E, Borriero C, Polletti S, Bedin F, Marenda M, Michieletto D, Mandana GM, Rodighiero S, Cuomo A, Natoli GGenes Dev , 2023 Nov-Dec; 37(21-24):1017-10402023 Nov-Dec
  • Kolev NG, Yario TA, Benson E, Steitz JAEMBO Rep , 2008 Oct; 9(10):1013-8. Epub 2008 Aug 82008 Oct