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> Rabbit Anti-DNMT1 Polyclonal Antibody

Rabbit Anti-DNMT1 Polyclonal Antibody

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Référence HPA002694-25ul

Conditionnement : 25ul

Marque : Atlas Antibodies

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HPA002694
DNA (cytosine-5-)-methyltransferase 1

Anti-DNMT1 Antibody

Polyclonal Antibody against HUMAN DNMT1

0.1 mg/ml
Anti-DNMT1 Antibody
Anti-DNMT1 Antibody
Anti-DNMT1 Antibody
Anti-DNMT1 Antibody
Anti-DNMT1 Antibody
Anti-DNMT1 Antibody
Anti-DNMT1 Antibody
Anti-DNMT1 Antibody
Anti-DNMT1 Antibody
Anti-DNMT1 Antibody
Anti-DNMT1 Antibody
info@biovalley.fr

Immunohistochemistry analysis in human placenta and pancreas tissues using HPA002694 antibody. Corresponding DNMT1 RNA-seq data are presented for the same tissues.

Product details

  • Target protein: DNA (cytosine-5-)-methyltransferase 1
  • Target gene: DNMT1
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000004099 (85%)RAT - ENSRNOG00000039859 (86%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000045526
  • Concentration: 0.1 mg/ml

Applications

  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:200 - 1:500
    • Retrieval method: HIER pH6

Validation

  • Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.  

Target information

  • Protein name: DNA (cytosine-5-)-methyltransferase 1
  • Gene name: DNMT1
  • Alternative gene names: CXXC9, DNMT, MCMT, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (5)

  • Zhang R, Gan W, Zong J, Hou Y, Zhou M, Yan Z, Li T, Lv S, Zeng Z, Wang W, Zhang F, Yang MFront Immunol None2023 Feb 22
  • Xiong Y, Hu L, Zhang T, Wang M, Xu H, Li TC, Sun Y, Wang CCJ Assist Reprod Genet , 2019 Nov 22; 37(1):33-43. Epub 2019 Nov 222019 Nov 22