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> Rabbit Anti-DNMT3B Polyclonal Antibody

Rabbit Anti-DNMT3B Polyclonal Antibody

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Référence HPA001595-25ul

Conditionnement : 25ul

Marque : Atlas Antibodies

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HPA001595
DNA (cytosine-5-)-methyltransferase 3 beta

Anti-DNMT3B Antibody

Polyclonal Antibody against HUMAN DNMT3B

0.1 mg/ml
Anti-DNMT3B Antibody
Anti-DNMT3B Antibody
Anti-DNMT3B Antibody
Anti-DNMT3B Antibody
Anti-DNMT3B Antibody
Anti-DNMT3B Antibody
Anti-DNMT3B Antibody
Anti-DNMT3B Antibody
Anti-DNMT3B Antibody
Anti-DNMT3B Antibody
Anti-DNMT3B Antibody
Anti-DNMT3B Antibody
Anti-DNMT3B Antibody
Anti-DNMT3B Antibody
Anti-DNMT3B Antibody
info@biovalley.fr

Immunohistochemistry analysis in human testis and skeletal muscle tissues using HPA001595 antibody. Corresponding DNMT3B RNA-seq data are presented for the same tissues.

Product details

  • Target protein: DNA (cytosine-5-)-methyltransferase 3 beta
  • Target gene: DNMT3B
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000027478 (62%)RAT - ENSRNOG00000010625 (66%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000050731
  • Concentration: 0.1 mg/ml

Applications

  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:200 - 1:500
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.  
  • Western Blot (WB)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. 

Target information

  • Protein name: DNA (cytosine-5-)-methyltransferase 3 beta
  • Gene name: DNMT3B
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (11)

  • Shokry D, Khan MW, Powell C, Johnson S, Rennels BC, Boyd RI, Sun Z, Fazal Z, Freemantle SJ, Parker MH, Vieson MD, Samuelson JP, Spinella MJ, Singh RCell Commun Signal , 2024 Oct 31; 22:528. Epub 2024 Oct 182024 Oct 31
  • Gondaliya P, Jash K, Srivastava A, Kalia KJ Diabetes Metab Disord , 2023 May 25; 22(2):1105-1115. Epub 2023 May 252023 May 25