Rabbit Anti-Hamster IgG (H+L), Rat/Mse SP ads-PE
Référence MBS674930-5x0,25mg
Conditionnement : 5x0.25mg
Marque : MyBiosource
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Rabbit Anti-Hamster IgG (H+L) Secondary Antibody – Rabbit Polyclonal
For Research Use Only. Not for use in diagnostic procedures. Catalog #: MBS674930MBS674930 is a secondary antibody targeting Rabbit Anti-Hamster IgG (H+L). Also known as Rabbit Anti-Hamster IgG (H+L), Rat/Mse SP ads-PE. Reactive in Hamster. Host: Rabbit. More details
Product Overview
Product Name
Rabbit Anti-Hamster IgG (H+L), Secondary Antibody
Full Product Name
Rabbit Anti-Hamster IgG (H+L), Rat/Mse SP ads-PE
Product Synonym Names
Rabbit Anti-Hamster IgG(H+L), Rat/Mse SP ads - Phycoerythrin-Labeled; Rabbit Anti-Hamster IgG (H+L) (Absorbed against mouse & rat serum proteins)
Clonality
Polyclonal
Isotype
IgG
Host
Rabbit
Species Reactivity
Hamster
Form/Format
R - phycoerythrin (R - PE) Conjugate
Purity/Purification
Affinity chromatography on hamster IgG covalently linked to agarose
Concentration
0.1 mg/ml (lot specific)
Specificity
Reacts with the heavy and light chains of Armenian and Syrian hamster IgG as demonstrated by ELISA and/or flow cytometry; may also react with the light chains of other hamster immunoglobulins; minimal cross reactivity with mouse and rat serum proteins
Preparation and Storage
The purified (UNLB) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of 100 mM borate buffered saline, pH 8.2. No preservatives or amine-containing buffer salts added. Store at 2- 8 degree C.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
The fluorescein (FITC) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The horseradish peroxidase (HRP) conjugate is supplied as 1.0 mL of stock solution in 50% glycerol/50% PBS, pH 7.4. No preservative added. Store at 2-8 degree C or long-term at -20 degree C.
The biotin (BIOT) conjugate is supplied as 0.5 mg in 1.0 mL PBS/NaN3. Store at 2-8 degree C.
The R-phycoerythrin (R-PE) conjugate is supplied as 0.1 mg in 1.0 mL of PBS/NaN3 and a stabilizing agent. Store at 2-8 degree C. Do not freeze!
The low endotoxin, azide-free (LE/AF) antibody is supplied as 0.5 mg purified immunoglobulin in 1.0 mL of PBS. Aliquot and store at or below -20 degree C.
Protect fluorochrome-conjugated forms from light. Reagents are stable for the period shown on the label if stored as directed.
Source
Pooled antisera from rabbits hyperimmunized with Syrian and Armenian hamster IgG
Cross Absorption
Mouse and rat serum proteins
Characterization
To insure lot-to-lot consistency, each batch of product is tested by ELISA and flow cytometry to conform to characteristics of a standard reference reagent.
Product Categories/Family for Rabbit Anti-Hamster IgG (H+L) secondary antibody
Applications Tested/Suitable for Rabbit Anti-Hamster IgG (H+L) secondary antibody
ELISA; FLISA; Immunofluorescence Staining
Application Notes for Rabbit Anti-Hamster IgG (H+L) secondary antibody
Characterization:
To insure lot-to-lot consistency, each batch of product is tested by ELISA or FLISA to conform to characteristics of a standard reference reagent.
Working Dilutions:
FLISA:
Flourescein conjugate 1:200 - 1:400
R-phycoerythrin conjugate 1ug/mL
ELISA:
Purified Antibody 1ug/mL
Biotin conjugate 1:5,000 - 1:20,000
Other Applications: Since applications vary, you should dtermine the optimum working dilution of the product that is approptiate for your specific need.
To insure lot-to-lot consistency, each batch of product is tested by ELISA or FLISA to conform to characteristics of a standard reference reagent.
Working Dilutions:
FLISA:
Flourescein conjugate 1:200 - 1:400
R-phycoerythrin conjugate 1ug/mL
ELISA:
Purified Antibody 1ug/mL
Biotin conjugate 1:5,000 - 1:20,000
Other Applications: Since applications vary, you should dtermine the optimum working dilution of the product that is approptiate for your specific need.
Other Notes
Small volumes of Rabbit Anti-Hamster IgG (H+L) secondary antibody vial(s) may occasionally become entrapped in the seal of the product vial during shipment and storage. If necessary, briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container`s cap. Certain products may require to ship with dry ice and additional dry ice fee may apply.