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> Rabbit Anti-KLF4 Polyclonal Antibody

Rabbit Anti-KLF4 Polyclonal Antibody

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Référence HPA002926

Conditionnement : 100ul

Marque : Atlas Antibodies

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HPA002926
Kruppel-like factor 4 (gut)

Anti-KLF4 Antibody

- ChIP-Certified

Polyclonal Antibody against HUMAN KLF4

0.2 mg/ml
Anti-KLF4 Antibody
Anti-KLF4 Antibody
Anti-KLF4 Antibody
Anti-KLF4 Antibody
Anti-KLF4 Antibody
Anti-KLF4 Antibody
Anti-KLF4 Antibody
Anti-KLF4 Antibody
Anti-KLF4 Antibody
Anti-KLF4 Antibody
Anti-KLF4 Antibody
Anti-KLF4 Antibody
Anti-KLF4 Antibody
Anti-KLF4 Antibody
Anti-KLF4 Antibody
Anti-KLF4 Antibody
Anti-KLF4 Antibody
Anti-KLF4 Antibody
Anti-KLF4 Antibody
info@biovalley.fr

Immunohistochemistry analysis in human skin and pancreas tissues using HPA002926 antibody. Corresponding KLF4 RNA-seq data are presented for the same tissues.

Product details

  • Target protein: Kruppel-like factor 4 (gut)
  • Target gene: KLF4
  • Verified species reactivity: Human
  • Interspecies information: Highest antigen sequence indentity to the following orthologs: MOUSE - ENSMUSG00000003032 (89%)RAT - ENSRNOG00000016299 (89%)
  • Clonality: Polyclonal
  • Isotype: IgG
  • Host: Rabbit
  • Buffer: 40% glycerol and PBS (pH 7.2). 0.02% sodium azide is added as preservative.
    Material Safety Data Sheet
  • Purification method: Affinity purified using the PrEST antigen as affinity ligand
  • Antigen sequence: Recombinant Protein Epitope Signature Tag (PrEST) antigen sequence
  • Notes: Gently mix before use. Optimal concentrations and conditions for each application should be determined by the user.

Current lot

  • Unit size: 100µl
  • Current lot: 000011564
  • Concentration: 0.2 mg/ml

Applications

  • Chromatin Immunoprecipitation (ChIP) recommended conditions
    • Working concentration:  1-10µg per reaction
  • Immunofluorescence in Cell Lines (ICC-IF) recommended conditions
    • Working concentration: 0.25-2 µg/ml
    • Fixation/Permeabilization: PFA/Triton X-100
  • Immunohistochemistry (IHC) recommended conditions
    • Dilution: 1:1000 - 1:2500
    • Retrieval method: HIER pH6
  • Western Blot (WB) recommended conditions
    • Working concentration: 0.04-0.4 µg/ml

Validation

  • Chromatin Immunoprecipitation (ChIP)Validated for use in ChIP-Exo-Seq and other ChIP-based assays via chromatin immunoprecipitation followed by λ exonuclease digestion and next-generation DNA sequencing to precisely map protein-DNA interactions at single base pair resolution. *Please note not all antibody batches are validated for use in ChIP applications. 
  • Immunofluorescence in Cell Lines (ICC-IF) 
  • Immunohistochemistry (IHC)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.  
  • Western Blot (WB)Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues. Recombinant expression validation in WB using target protein overexpression. 

Target information

  • Protein name: Kruppel-like factor 4 (gut)
  • Gene name: KLF4
  • Alternative gene names: EZF, GKLF, 
  • Ensembl: 
  • Entrez: 
  • UniProt: 

Shipping and storage

  • Shipping: Normally shipped at ambient temperatureStorage: Store at +4°C for short term storage. Long time storage is recommended at -20°C.

References (16)

  • Oppenheim O, Giese W, Park H, Baumann E, Ivanov A, Beule D, Eichmann A, Gerhardt HbioRxiv , . Epub 2025 Jan 32025 Jan 3
  • Schaal DL, Amucheazi AA, Jones SC, Nkadi EH, Scott RSJ Virol , 2024 Sep 18; 98(10):e00995-24. Epub 2024 Sep 182024 Sep 18

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Référence
Description
Cond.
Prix HT
HPA002926-25ul
Rabbit Anti-KLF4 Polyclonal Antibody
 25ul