Rabbit Anti-LTF Polyclonal Antibody
Référence AP8698A
Conditionnement : 400ul
Marque : Abcepta
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Téléphone : +1 850 650 7790
LTF Antibody
Affinity Purified Rabbit Polyclonal Antibody (Pab)
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Western blot analysis of LTF Antibody (Cat. #AP8698a) in MDA-MB231 cell line lysates (35ug/lane). LTF (arrow) was detected using the purified Pab.
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Western blot analysis of LTF Antibody (Cat. #AP8698a) in mouse spleen tissue lysates (35ug/lane). LTF (arrow) was detected using the purified Pab.
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All lanes : Anti-LTF Antibody at 1:1000-2000 dilution Lane 1: Human plasma tissue lysate Lane 2: Hela whole cell lysate Lane 3: Human uterus tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-LTF Antibody at 1:1000 dilution Lane 1: Human plasma tissue lysate Lane 2: Human fetal kidney tissue lysate Lane 3: Human uterus tissue lysate Lane 4: Human skeletal muscle tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-LTF Antibody at 1:4000 dilution Lane 1: A549 whole cell lysate Lane 2: Human plasma tissue lysate Lane 3: Human fetal kidney tissue lysate Lane 4: Human breast tissue lysate Lane 5: Human uterus tissue lysate Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 78 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
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LTF Antibody (Cat. #AP8698a) flow cytometric analysis of MDA-MB231 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of LTF Antibody (Cat. #AP8698a) in MDA-MB231 cell line lysates (35ug/lane). LTF (arrow) was detected using the purified Pab.
Application 
| FC, WB, E |
|---|---|
| Primary Accession | P02788 |
| Reactivity | Human, Mouse |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Calculated MW | 78182 Da |
| Gene ID | 4057 |
|---|---|
| Other Names | Lactotransferrin, Lactoferrin, 3421-, Growth-inhibiting protein 12, Talalactoferrin, Lactoferricin-H, Lfcin-H, Kaliocin-1, Lactoferroxin-A, Lactoferroxin-B, Lactoferroxin-C, LTF, GIG12, LF |
| Target/Specificity | This LTF antibody is generated from rabbits immunized with LTF recombinant protein. |
| Dilution | FC~~1:10~50 WB~~1:4000 E~~Use at an assay dependent concentration. |
| Format | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Precautions | LTF Antibody is for research use only and not for use in diagnostic or therapeutic procedures. |
For Research Use Only. Not For Use In Diagnostic Procedures.
| Name | LTF (HGNC:6720) |
|---|---|
| Synonyms | GIG12, LF |
| Function | Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. |
| Cellular Location | [Isoform 1]: Secreted. Cytoplasmic granule. Note=Secreted into most exocrine fluids by various endothelial cells. Stored in the secondary granules of neutrophils |
| Tissue Location | High levels are found in saliva and tears, intermediate levels in serum and plasma, and low levels in urine. In kidney, detected in the distal collecting tubules in the medulla but not in the cortical region or in blood vessels. Detected in peripheral blood neutrophils (at protein level). Isoform 1 and isoform DeltaLf are expressed in breast, prostate, spleen, pancreas, kidney, small intestine, lung, skeletal muscle, uterus, thymus and fetal liver Isoform 1 is expressed in brain, testis and peripheral blood leukocytes; isoform DeltaLf is barely detectable in these tissues Isoform DeltaLf is expressed in placenta, liver and ovary; isoform 1 is barely detectable in these tissues. In kidney, isoform 1 is expressed at high levels in the collecting tubules of the medulla but at very low levels in the cortex. |
- Primary Antibodies
- Cancer
- Cardiovascular
- Signal Transduction
| Author : Zhao Liu 1, Chen Zhang 2, Beiming Cui 3, Yijie Wang 4, Kaisheng Lim 5, Kai Li 6, Jean Paul Thiery 7, Jun Chen 8, Chun Loong Ho 9 Biomed Pharmacother .2023 Feb 28;161:114443. doi: 10.1016/j.biopha.2023.114443. Online ahead of print. Application: WB | 36863098 |
Provided below are standard protocols that you may find useful for product applications.
Background
LTF is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity, making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties, including regulation of iron homeostasis, host defense against a broad range of microbial infections, anti-inflammatory activity, regulation of cellular growth and differentiation and protection against cancer development and metastasis.